Distinguished Professor
James Dale
Faculty of Science,
School of Biology & Environmental Science
Personal details
Positions
- Professor
Faculty of Science,
School of Biology & Environmental Science
Keywords
Biofortification of bananas for Africa, Geminivirus and nanovirus (BBTV) resistance, Bananas resistant to Fusarium wilt Tropical Race 4, Molecular farming - novel proteins and bioreactors, Genetic engineering of bananas, potatoes, sugarcane, Control of transgene expression and stability in plants
Research field
Agricultural biotechnology, Biochemistry and cell biology, Microbiology
Field of Research code, Australian and New Zealand Standard Research Classification (ANZSRC), 2020
Qualifications
- PhD (University of Sydney)
- BScAgr (University of Sydney)
Experience
Distinguished Professor James Dale is the inaugural Director of the Centre for Tropical Crops and Biocommodities (CTCB) at Queensland University of Technology (QUT) which incorporates Tropical Crop Biotechnology, the Syngenta Centre for Sugarcane Biofuels Development and Sugar Research and Innovation (SRI). The CTCB consists of around 50 researchers as well as more than 25 postgraduate students. It is a horizontally integrated, multidisciplinary centre focusing on tropical crops with expertise from gene discovery and genetic modification through to biomass fractionation, processing and value adding. Its facilities range from state-of-the-art molecular biology laboratories through small scale biomass processing labs, PC2 plant growth facilities and glasshouses to a major biomass pilot plant. The CTCB has collaborative projects in Africa, Asia, Europe and North and South America. Prof Dale has been involved in biotechnology research for more than 30 years with specific interests in (i) the biofortification of bananas, (ii) molecular farming for high value medical proteins, including vaccines, in tobacco and bioethanol from sugarcane, and (iii) the development of disease resistance in genetically modified bananas, papaya and sugarcane. He and his research team have developed a range of biotechnology methods and products for expressing transgenes in plants. He has also led research and development programs in the international arena most of which have been based in South East Asia and the South Pacific (Vietnam, Thailand, Malaysia, Tonga, Fiji and PNG) and more recently in Africa (Uganda, Kenya and Tanzania). Prof Dale has published more than 80 research papers, reviews and book chapters. He an inventor on 9 granted patents or patent applications of which to date the most successful has been the GeneCo technology which was sold to Affymetrix, the USA based leading DNA chip maker. He has been a member of the Australian Research Council’s College of Experts (2004-2006) and Chair of the Biological Sciences and Biotechnology Panel (2006), and has been a member of the Gene Technology Technical Advisory Committee (GTTAC) of the Office of the Gene Technology Regulator (2001-2007) and the Queensland Biotechnology Advisory Council. He was the founder and chief scientific officer of Farmacule Bioindustries, Australia’s first molecular farming company. Farmacule merged with the ASX listed company, Aquacarotene, to form the first listed molecular farming company in Australia and the new company was named Leaf Energy. In 2008, QUT and Syngenta AG formed a new collaborative centre, the Syngenta Centre for Sugarcane Biofuels Development, based within the CTCB at QUT. This centre is staffed by both QUT and Syngenta scientists with the objective of developing economically competitive cellulosic ethanol using genetically modified sugarcane. Prof Dale is leading this initiative. Prof Dale is also leading a major international Grand Challenges in Global Health Project funded by the Bill and Melinda Gates Foundation to develop biofortified bananas for East Africa with potential further application in Asia. The project is a close collaboration between QUT and the National Agricultural Research Organisation of Uganda. Where appropriate, the biofortification trait is being combined with disease resistance to increase both yield and farmer adoption. The first Australian GM banana field trials, which include biofortified or disease resistant bananas, commenced in early 2009 and harvesting commenced in early 2010. There are already multiple lines with greater than the target level of pro-vitamin A. The first Ugandan field trial from this program commenced in 2010. This is possibly the first trial in sub-Saharan Africa of any GM crop where the events were generated in Africa by a national lab. In late 2012, the project was refunded for Phase 3 moving from the research phase to the development phase. The project was further expanded on 2012 into India through a collaboration with the Indian Department of Biotechnology. In 2004, Prof Dale was made an Officer in the Order of Australia (AO) for services to agricultural biotechnology. In 2008, he was appointed as QUT’s second Distinguished Professor.
Publications
- Dugdale, B., Mortimer, C., Kato, M., James, T., Harding, R. & Dale, J. (2014). Design and construction of an in-plant activation cassette for transgene expression and recombinant protein production in plants. Nature Protocols, 9(5), 1010–1027. https://eprints.qut.edu.au/70642
- Harrison, M., Geijskes, J., Lloyd, R., Miles, S., Palupe, A., Sainz, M. & Dale, J. (2014). Recombinant cellulase accumulation in the leaves of mature, vegetatively propagated transgenic sugarcane. Molecular Biotechnology, 56(9), 795–802. https://eprints.qut.edu.au/70998
- Dugdale, B., Mortimer, C., Kato, M., James, T., Harding, R. & Dale, J. (2013). In plant activation: An inducible, hyperexpression platform for recombinant protein production in plants. The Plant Cell, 25(7), 2429–2443. https://eprints.qut.edu.au/62398
- Mlalazi, B., Welsch, R., Namanya, P., Khanna, H., Geijskes, J., Harrison, M., Harding, R., Dale, J. & Bateson, M. (2012). Isolation and functional characterisation of banana phytoene synthase genes as potential cisgenes. Planta, 236(5), 1585–1598. https://eprints.qut.edu.au/52937
- Paul, J., Becker, D., Dickman, M., Harding, R., Khanna, H. & Dale, J. (2011). Apoptosis-related genes confer resistance to Fusarium wilt in transgenic 'Lady Finger' bananas. Plant Biotechnology Journal, 9(9), 1141–1148. https://eprints.qut.edu.au/47028
- Peraza Echeverria, S., Dale, J., Harding, R., Smith, M. & Collet, C. (2008). Characterization of disease resistance gene candidates of the nucleotide binding site (NBS) type from banana and correlation of a transcriptional polymorphism with resistance to Fusarium oxysporum f.sp. cubense race 4. Molecular Breeding, 22(4), 565–579. https://eprints.qut.edu.au/17102
- Ha, V., Coombs, S., Revill, P., Harding, R., Vu, M. & Dale, J. (2008). Molecular characterization of begomoviruses and DNA satellites from Vietnam: additional evidence that the New World geminiviruses were present in the Old World prior to continental separation. Journal of General Virology, 89(1), 312–326. https://eprints.qut.edu.au/17096
- Khanna, H., Paul, J., Harding, R., Dickman, M. & Dale, J. (2007). Inhibition of Agrobacterium-Induced Cell Death by Antiapoptotic Gene Expression Leads to Very High Transformation Efficiency of Banana. Molecular Plant-Microbe Interactions, 20(9), 1048–1054. https://eprints.qut.edu.au/9017
- Pecheniuk, N., Marsh, N., Walsh, T. & Dale, J. (1997). Use of First Nucleotide Change Technology to Determine the Frequency of Factor V Leidon in a Population of Australian Blood Donors. Blood Coagulation and Fibrinolysis, 491–495.
- Burns, T., Harding, R. & Dale, J. (1995). The genome organization of banana bunchy top virus: analysis of six ssDNA components. Journal of General Virology, 76(6), 1471–1482.
QUT ePrints
For more publications by James, explore their research in QUT ePrints (our digital repository).
Awards
- Type
- Appointment to State/National/International Reference Group or Government Committees
- Reference year
- 2015
- Details
- Chair of the Australian Research Council's Biotechnology and Biological Sciences Panel; member of the ARC College of Experts from 2004 to 2006.
- Type
- Fellowship of a Learned Academy or Membership of AIATSIS
- Reference year
- 2015
- Details
- Elected Fellow of ATSE
- Type
- Other
- Reference year
- 2014
- Details
- TIME Magazine Top 25 Inventions of 2014
- Type
- Academic Honours, Prestigious Awards or Prizes
- Reference year
- 2004
- Details
- Appointed as Officer of the Order of Australia (AO) for services to agricultural biotechnology
Selected research projects
- Title
- Production of Novel, Exogenous Proteins in Sugarcane
- Primary fund type
- CAT 1 - Australian Competitive Grant
- Project ID
- Farm01
- Start year
- Keywords
- Sugarcane; Biofactory
- Title
- Optimization of Transgene Expression in Sugarcane
- Primary fund type
- CAT 1 - Australian Competitive Grant
- Project ID
- LP0990296
- Start year
- 2010
- Keywords
- Sugarcane; Gene Expression; Plant Biotechnology; Plant Improvement
- Title
- Extreme Expression: Building a Platform for Industrial Plant Biotechnology
- Primary fund type
- CAT 1 - Australian Competitive Grant
- Project ID
- FS100100083
- Start year
- 2011
- Keywords
- Biofarming; Transgene Expression; Silencing; Inducible Expression
- Title
- Generation of Bananas with Resistance to Fusarium Wilt
- Primary fund type
- CAT 1 - Australian Competitive Grant
- Project ID
- LP110100186
- Start year
- 2011
- Keywords
- Fusarium Wilt; Apoptosis Genes; R Genes; Banana
- Title
- A platform for the continuous genetic improvement of accepted cultivars of vegetatively propagated horticultural crops (HAL)
- Primary fund type
- CAT 1 - Australian Competitive Grant
- Project ID
- AI13008
- Start year
- 2013
- Keywords
- Bananas; Cisgenics; Intergenerational Transgene Stability; Marker Free Transformation; Potatoes
Projects listed above are funded by Australian Competitive Grants. Projects funded from other sources are not listed due to confidentiality agreements.
Supervision
Completed supervisions (Doctorate)
- Strategies for Improved Disease Resistance in Micro-propagated Bananas (2013)
- Viruses of Banana in East Africa (2012)
- Towards the Biofortification of Banana Fruit for Enhanced Micronutrient Content (2011)
- Defining the role of phytoene synthase in carotenoid accumulation of high provitamin A bananas (2010)
- Recombinant protein production using a Tobacco yellow dwarf virus-based episomal expression vector: control of Rep activity (2009)
- The Manipulation of Apoptosis-Related Genes to Generate Resistance to Fusarium Wilt and Water Stress in Banana (2009)
- Detection and Identification of Potyviruses and Geminiviruses in Vietnam (2007)
- Molecular Cloning and Characterisation of Potential Fusarium Resistance Genes in Banana (Musa acuminata ssp. malaccensis) (2007)
- Gene function in Banana Bunchy Top Virus (2000)
- Promoter Activity Associated with the Intergenic Regions of BBTV (1999)
The supervisions listed above are only a selection.