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Uncovering the dark transcriptome and proteome with long-read sequencing

Parts of the transcriptome and proteome are invisible to current technologies. We refer to them as the dark transcriptome and proteome and they encompass uncharacterised mRNA and protein isoforms. Importantly, this hidden molecular phenotype is believed to play a critical role in animal health and behavior.Recent developments in mRNA analytical methods such as long-read sequencing (PacBio, ONT) have enabled better characterization of isoforms and are redefining the transcriptome landscape in many species. We and others have found that data generated …

Study level
Honours
Faculty
Faculty of Science
School
School of Biology and Environmental Science

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