BIOM05 The functional role of IRX cluster proteins in prostate cancer stem cells

Overview

Prostate cancer is a leading cause of cancer-associated death among Australian men. Unfortunately, there is no cure for advanced prostate cancer patients who develop resistance to currently available treatments. Cancer stem cells are a significant factor contributing to treatment resistance in prostate cancer. The Iroquois-class homeodomain family, consisting of six Iroquois (IRX) genes, encodes transcription factors that have been implicated in cancers. Our previous studies1 identified a link between IRX4, one of the IRX cluster genes, and prostate cancer stemness. However, the roles and mechanisms of action of the other IRX genes in this context remain unknown. In this project, we aim to elucidate their functions by developing a prostate cancer stem cell-enriching model and conducting follow-up functional studies. This research will provide valuable insights into the roles of IRX genes in prostate cancer and could potentially lead to the development of new therapeutic strategies for advanced prostate cancer patients.

Research engagement

This project may utilise a literature review and a range of in vitro prostate cancer cell line-based techniques, including qPCR, RT-PCR, western blot, cell culture, cloning techniques, cell transfection, cell proliferation assay, cancer stem cell assays, plasmid isolation, RNA isolation, co-immunoprecipitation, and more. The student is expected to actively observe, learn, and eventually conduct experiments under supervision.

Research activities

The student is expected to train in the above techniques under the guidance of supervisors. This includes learning statistical methods, data analysis, techniques for assessing cancer stemness, cell culture proficiency, and molecular biology techniques. Additionally, the student will develop scientific writing skills for proposals and reports, conduct comprehensive literature reviews, and hone data presentation skills for oral presentations and writing reports to the research team.

Outcomes

1. Association analysis of the expression of IRX cluster genes with prostate cancer therapy response and resistance.

2. Evaluate the effect of selected IRX genes on prostate cancer stemness.

3. Stem cell functional assays with selected IRX overexpression and knockdown Models.

Skills and experience

Experience in mammalian cell culture is beneficial but not necessary. However, students should have an interest in acquiring these technical skills. Knowledge of basic biochemistry, cell and molecular biology, and basic laboratory techniques will be an advantage.

Start date

1 November, 2024

End date

21 February, 2025

Location

Translational Research Institute

Keywords

• Iroquois cluster genes
• Cancer stem cells
• Prostate cancer

Contact

a.vitharanage@qut.edu.au