About us

We provide specialist equipment and expert scientists to help you carry out analytical research.

Our state-of-the-art equipment and expert technologists offer transdisciplinary analytical support, including sample preparation, data collection and interpretation of results.

The Central Analytical Research Facility (CARF) is located across QUT campuses, with the main node situated in the Science and Engineering Centre at our Gardens Point campus.

Services and equipment

We offer services to QUT staff and students, external researchers and commercial organisations, ranging from basic technical support to commercial research.

Specialist staff are available to carry out analyses, or we can train you to use the instruments to do your own work.

Prices for services and equipment use are current as at time of publishing, and are subject to change.

Contact carf@qut.edu.au to discuss your project.

Capabilities

We support scientific analytical research, teaching and learning to enable real-world results.

Cell analysis

The Cell Analysis Facility offers state-of-the art imaging and cell analysis instrumentation to facilitate imaging and analysing the molecular and structural organisation of cells, tissues, and bioengineered materials.

The facility is run by qualified operators and provides comprehensive training in all aspects of microscopy and cell analysis including experimental design, sample preparation, instrument operation and data analysis.

Technologies and equipment offered

  • Confocal laser scanning microscopy
  • Wide-field fluorescence microscopy
  • Live-Cell Imaging
  • Transmitted-light imaging (Phase Contrast, Differential Interference Contrast)
  • Specialized microscopy (FRAP, FRET)
  • Scanning electron microscopy - Back Scatter Electron Detection
  • Flow cytometry
  • Digital Spatial Profiling – Nanostring GeoMx Digital Spatial Profiler
  • nCounter Analysis System for Multiplex Analysis up to 800 Targets - Nanostring - nCounter Prep station and Digital Analyser

Services offered

The Cell Analysis Facility provides expertise and technical resources to assist researchers in designing experimental plans, acquiring appropriate data, and interpreting data. Training is provided for staff and students to independently conduct their cell analysis research. For the infrequent, untrained user, our facility provides a completely assisted technical support service.

Contact us

For more information on what we offer and how to access our expertise please email cellanalysisfacility@qut.edu.au

Microscopes

The Cell Analysis Facility provides access to a wide range of microscope platforms including both routine and advanced optical and electron microscopes. The technology capabilities include scanning electron microscopy, bright field microscopy (phase contrast and DIC), multi-channel fluorescence microscopy, confocal laser scanning microscopy, and is fully equipped for live-cell imaging.

Instrument Capabilities and features
Evident FluoView4000
  • Laser scanning confocal microscope
  • IXplore inverted stand
  • High resolution: 'optical slicing' of samples, Z-stack
  • Laser excitation: 10 laser lines (405, 445, 488, 514, 561, 594, 640, 685, 730 and 785 nm)
  • 4 tunable spectral detectors (SilVIR) and 1 transmitted light detector
  • High resolution objectives
  • Fast galvo scanners and high speed resonant scanner available
  • Environmental chamber for the control of temperature, CO2 concentration, and humidity guarantees ideal conditions for prolonged time-lapse imaging
  • Imaging diverse sample formats from cultured cells on 2D and 3D structures
Leica AF6000LX Widefield Microscope
  • Fluorescence imaging
  • DMI6000 inverted stand
  • Long working distance 10x, 20x and 40x dry, and 63x glycerol objectives
  • Differential Interference Contrast (DIC)
  • External filter wheel for fast multi-colour acquisition
  • X-Y-Z motorised stage adaptable to a variety of sample formats including multi-well and other cell culture plates and microscopy slides
  • Tiling of large samples with excellent stitching of image sets.
  • Live-cell time lapse imaging in DIC and Fluorescence of multiple sites in parallel
  • Environmental chamber for the control of temperature, CO2 concentration, and humidity guarantees ideal conditions for prolonged time-lapse imaging
Zeiss AxioImager Z2 Widefield Microscope
  • Fully automated research microscope with fluorescence
  • Transmitted Light DIC
  • Epi-fluorescence Imaging: DAPI, FITC, CY3, Texas Red and Cy5
  • 3D z stacks
  • Tilescan and Stitching
  • Deconvolution
  • Apotome
Holomonitor M4 Live Cell Imaging System
  • Non-invasive, label-free Live Cell Imaging System
  • Provides quantitative data on single-cell level
  • Assays include Cell QC, Single Cell Tracking, Cell Counter, Cell Motility, Kinetic Morphology, Cell Proliferation and Wound Healing
Nikon Eclipse TiS Inverted Microscope with Fluorescence
  • Epi-fluorescence imaging: DAPI, FITC, Cy3 and Cy5 filter cubes
  • Transmitted light - Phase Contrast
  • NIS-Br imaging software
Nikon Eclipse Ts2 Routine Inverted Microscope
  • LED illumination for Transmitted Light and Epi-fluorescence imaging
  • DAPI, FITC and TRITC filter cubes
  • Phase Contrast
Hitachi TM3000 ESEM
  • Benchtop Scanning Electron Microscope: no sample coating required
  • Backscattered imaging at 5 or 15kV beam energy
  • Charge-up reduction mode for insulating samples

Indicative Pricing

Prices are indicative and vary depending on whether work is for external researchers or commercial organisations, and who carries out the work. We will provide a costing proposal for your approval before any work starts.

Indicative pricing for external researchers

Equipment, service or analysis with technician assistanceUnassisted
Evident FluoView4000 $175.00 $80.00

Flow cytometry

The flow cytometry resources provide multi-parameter flow cytometric analysis for the evaluation of cell proliferation and development, apoptosis, presence of internal and external cell markers and proteins of interest, cell cycle analysis and much more.

Instrument Capabilities and features
BD FACSCelesta Flow Cytometer
  • High-speed, multi-colour, digital analyzer
  • 3 lasers (405, 488 and 561nm)
  • 12 detectors
  • High Throughput Sampler
  • Software: FACSDiva - allows for automatic compensation setup and allows for compensation after data acquisition. This minimizes the amount of sample needed to obtain correct compensation before data acquisition.
MACSQuant Flow Cytometer
  • High-speed, multi-colour, digital analyzer
  • 3 Lasers (405, 488 and 640nm)
  • 10 Detectors
  • Mini Sampler for tube and 96-well plate automation.
BD FACSMelody Fluorescence Activated Cell Sorter
  • Installed in a fully integrated biosafety cabinet (BSC) with built in Aerosol management system
  • 3 Lasers (405, 488 and 561nm)
  • 11 detectors (2 scatter parameters (FSC and SSC) and 9 Fluorescence Parameters
  • Sample Temperature Control.
FlowJo
  • Flexible and automatable analysis software for flow cytometry data.

Our experienced staff can provide training, advice and guidance on the experimental process and the analysis of results, to ensure optimal outcomes.

Spatial genomics

The Nanostring GeoMx DSP and nCounter Flex System are available through the CARF Cell Analysis Facility.

Instrument Capabilities and features
Nanostring nCounter Flex System
  • Supports applications for gene expression analysis, copy number analysis, miRNA profiling and analysis of RNA/protein biomarkers.
  • Ability to detect RNA, miRNA and DNA from a variety of tissue (fresh, frozen, formalin-fixed, Paraffin-embedded (FFPE), cell lysates, blood, saliva and single cells.
  • Up to 800 targets can be detected in a single reaction.
  • Assay.
  • For more information about nCounter assays, please visit Nanostring.
Nanostring GeoMx Digital Spatial Profiler

Digital spatial profiling (DSP) allows you to combine high-plex gene expression profiling with the spatial resolution of immunohistochemistry. This technology is useful for examining cellular interactions, tissue heterogeneity, pathogenicity and response to therapy.

The GeoMx DSP workflow starts with staining of the tissue section with up to four fluorescent markers to visualize the tissue morphology and detection probes coupled to photocleavable target specific oligonucleotide tags. The tissue section is imaged and regions of interest (ROI) are selected for expression profiling, based on the morphology of the tissue. The ROIs are sequentially exposed to UV light, releasing the oligonucleotide tags, which are quantitated on the Nanostring nCounter instrument or through next generation sequencing. The counts of each target are mapped back to the tissue location, revealing the spatial expression patterns of the tissue.

Supported Assays:

  • Whole Transcriptome Atlas (Human or Mouse) 19 000+ mRNA targets
  • Cancer Transcriptome Atlas (Human) 1830+ mRNA targets
  • Protein Assay with nCounter or NGS readout (Human or Mouse)

The protein assays and Immune pathways RNA panel use nCounter to quantitate the oligonucleotide tags whereas the larger CTA and WTA panels use next generation sequencing as read-out method.

Sample requirements:

  • FFPE or Fresh/Frozen tissue
  • Human and Mouse samples currently supported by Nanostring Assays

Recommended section thickness is 5um.

Since DSP experiments are complex, we require an initial consultation with you so that we may assist you with planning and design of your experiment. During this initial project discussion, we will gain an understanding of your project goals, sample type, desired analyte (protein/RNA), timeline and estimated cost. The major determinants of overall project cost will be the number of desired slides to be processed, region of interest (ROI) number and size, and nCounter/sequencing requirements. We are committed to working with your to execute y our spatial profiling experiments efficiently and cost-effectively.

Please contact us at spatialgenomics@qut.edu.au to discuss your project requirements.

Our experienced staff can provide training, advice and guidance on the experimental process and the analysis of results, to ensure optimal outcomes.

Booking instructions

Contact Christina Theodoropoulos via email at cellanalysisfacility@qut.edu.au to discuss how the Cell Analysis Facility can assist in your research, and to make a booking.

External users

Cell analysis facilities are available by arrangement to staff and postgraduate students from any university and other external users. Users external to QUT can access the facility from Monday to Friday, 9am-5pm. Contact us at cellanalysisfacility@qut.edu.au to discuss your requirements and to request a quote.

Our Cell Analysis Facility is available to QUT staff and students and external clients at competitive rates.

Elements and isotopes

We have equipment to chemically analyse a wide range of gas, liquid and solid samples.

Overview

Our researchers can help analyse your materials and samples using the cutting-edge technologies in our elements and isotopes laboratory.

Our laboratory houses an array of top-end instruments for chemically analysing a wide range of gas, liquid and solid samples, down to parts per trillion in some cases.

Most samples need to be crushed, powdered, extracted or diluted while avoiding contamination into a form that can be analysed by the instrument. We can assist with preparing samples as required.

Details

Our state-of-the-art equipment allows for a range of analyses and discovery.

Carbon, nitrogen and sulfur (CNS) analysis

Instrument Capabilities and features
LECO CNS928 Series Macro Determinator Analysis of carbon, nitrogen and sulfur in macro samples (up to 3g for nitrogen; up to 1.5g for carbon/nitrogen; up to 0.3g for carbon/nitrogen/sulfur).
FlashSMART CHNS or O Analysis of hydrogen, carbon, nitrogen, oxygen and sulfur in micro samples (maximum size 2mg).
Shimadzu TOC-V (with TNM attachment) Total Organic Carbon Analyser Measurement of total carbon (TC), inorganic carbon (IC), total organic carbon (TOC) and total nitrogen (TN) in liquid samples, such as river, lake, ocean, wastewater and industrial samples (max concentration ~100ppm).

Chromatography

Instrument Capabilities and features
Agilent 7890A and Shimadzu 2014 Gas Chromatograph Analysis of methane, carbon dioxide and nitrous oxide
Shimadzu Nexus GC-2030 Analysis of greenhouse gas and other permanent gases
Dionex Ion Chromatograph with Combustion capability Anion analysis (F-, Cl-, NO3-, SO42-, Br-) of aqueous samples, and solid samples (F-, Cl-, Br-, SO42-). Analysis methods can be made to order.

Electrochemistry

Instrument Capabilities and features
TPS pH, conductivity and dissolved oxygen meter and ion probes pH, conductivity, dissolved oxygen and specific ion measurement for aqueous samples.
Mettler Toledo T50 Titrator-Rondolino Automated titration with nine-place sample changer for alkalinity, acid base titration, etc.

Mass spectrometry

Instrument Capabilities and features
Sercon 20-22 Isotope Ratio Mass Spectrometer Precise measurements of 12C, 13C, 14N, 15N isotopes in gas, liquid or solid samples.
Agilent 8800 Inductively Coupled Plasma Mass Spectrometer (ICP-MS) Triple quadrupole mass spectrometer capable of trace metal analysis down to parts per trillion.
Agilent 8900 Laser Ablation Inductively Coupled Plasma Mass Spectrometer (LA-ICP-MS) Determination of trace element concentrations, down to parts per billion, in a wide range of solid samples.
Syft Technologies VOICE200ultra Selected Ion Flow Tube Mass Spectrometer (SIFT-MS) High sensitivity, real-time high-throughput quantitative gas analysis. Simultaneous analysis of chemically diverse VOCs and inorganics to pptv level.
Shimadzu TD-GCMS Thermal Desorption Gas Chromatograph Mass spectrometer Analysis of VOCs collected in thermal desorption tubes. Shimadzu TD-30 series.

Spectroscopy

Instrument Capabilities and features
Thermo Fisher Gallery Discrete Analyser Automated wet chemistry analyser with extensive applications: water, wastewater, soil, plant tissue, fertiliser, etc.
Agilent Cary 60 UV-VI Spectrophotometer Spectrometer for ultraviolet-visible analysis. Software allows for a wide range of applications.
Perkin Elmer Optima 8300 DV and Agilent 5800 Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) Elemental analysis down to parts per million. Dual view instrument for TDS up to 20% and dynamic linear range.
Los Gatos Research (LGR) Liquid Water Isotope Analyser Measures δ18O and δ2H water in liquid.

Sample preparation

Instrument Capabilities and features
Freeze Dryer Drying/water removal from solid samples including sediments, plant material and soils.
Bench top preparation Bench area for sample and reagent preparation. Glassware, ovens, dessicators, sinks and gases.
Dionex ASE 350 Accelerated Solvent Extractor (ASE) Extraction of solid and semi-solid samples using common solvents at elevated temperatures (40-200°C) and pressures.
Katanax electronic fusion instrument Preparation of inorganic samples (fusion solutions and fused disks) for X-ray fluorescence (XRF).
Milestone Ultrawave Single Reaction Chamber (SRC) SRC microwave digestion system, up to 199 bars of pressure and 350°C.
Clean room Trace metal analysis preparation.
Analab CleanAcids and Savillex DST-1000 Acid Purification System Sub-boiling stills for purification of acids and reagents such as HNO3, HCI, HF and other solvents.
Balance room Various analytical balances with precision to 0.1 mg and 0.01 mg, as well as an anti-static kit.

Services and equipment hire

Our equipment is available to staff, students, external researchers and commercial organisations at a competitive price. Our laboratory provides both self-service and full-service options.

Conditions

  • A minimum number of samples applies depending on the instrument or analysis. Extra charges may apply or waiting time may be longer to make a batch.
  • For non-conventional samples requiring non-routine analysis, method development or additional advice on analytical setup and data processing, an additional charge will apply.
  • Charges will apply for additional sample preparation required before analysis (eg. ICPMS acid digestion, LECO dry and grind, IC extraction).

Indicative pricing for external researchers

Equipment, service or analysis With technician assistanceUnassisted
Laser ablation ICP-MS
ICP-MS equipment use (half-day, 4 hours) $1,195 $1,005
ICP-MS equipment use (full-day, 8 hours) $1,790 $1,425
ICP analysis (per sample)
ICPOES - Major and trace element $35 N/A
ICPMS - Trace and ultra trace elements $60 N/A
Acid digestions/extractions for ICP analysis (per sample)
Room temperature extraction/dilution $12 N/A
Hotblock extraction/digestion $22 N/A
Hot plate digestion $80 N/A
Microwave digestion $125 N/A
Elemental analysis (per sample)
Elemental analysis: O (mg-sized samples) $45 N/A
Elemental analysis: CHNS (mg-sized samples) $45 N/A
Bulk elemental analysis: CNS (g-sized samples) $20 $15
Acid treated (IC removal) plus TOC analysis $30 $25
TOC-TN (liquid) $20 N/A
Sample preparation (per sample)
Dionex ASE (solvent extraction) $40 $20
Ashing $20 N/A
Sample Grinding and drying (~20g) $25 N/A
Fine milling for CHNS or O (< 1g) $12 N/A
Freeze drying (fit inside 50ml vial) $20 N/A
Nutrient analysis (per sample)
Gallery Discrete Analyser (NO3- and NH4+) $15 $10
Anions by IC (per sample)
Dionex 2100: F-, Cl-, NO3-, SO42-, Br- $25 $20
Sample dilution prep inculding analysis IC $30 $25
Integrion IC with Combustion for solids F-, Cl-, Br-, SO42- $50 N/A
Gas analysis (per sample)
CO2, CH4, N2O by GC $8 $7
Sample dilution prep plus analysis GHG $10 $9
H2, O2, CO, CO2, N2, N2O2, NO by GC $15 $12
Evacuated vial rental $1 $1
SIFT-MS
VOICE200 Ultra (half day, 4 hours) $575 $460
VOICE200 Ultra (full day, 8 hours) $1,000 $800
Stable Isotopes Analysis (per sample)
IRMS: N2O, N2, CO2 gas by Cryo-IRMS $11 $10
IRMS: C and N in solids by EA-IRMS $20 $15
2H and 18O by LWIA $20 $15

Indicative pricing for commercial organisations

Equipment, service or analysis With technician assistance
Laser ablation ICP-MS
ICP-MS equipment usage (half-day, 4 hours) $2,895
ICP-MS equipment usage (full-day, 8 hours) $4,320
SIFT-MS
Batch set-up fee $175 per batch
Known analytes (up to 10 compounds) $115 per sample
Unknown analytes (up to 10 compounds) $250 per sample
With operator (half day, 4 hours) $1,400
With operator (full day, 8 hours) $2,445
ICP analysis (per sample)
ICPOES - major and trace elements $60 per sample
ICPMS - trace and ultra trace elements $95 per sample
Acid digestions/extractions for ICP analysis (per sample)
Room Temperature extraction/dilution $25 per sample
Hot block extraction/digestion $45 per sample
Hot plate digestion $120 per sample
Microwave digestion $195 per sample
Elemental analysis
Elemental analysis: O (mg-sized samples) $100 per sample
Elemental analysis: CHNS (mg-sized samples) $100 per sample
Bulk elemental analysis: CNS (g-sized samples) $45 per sample
Acid treated (IC removal) plus TOC analysis $55 per sample
TOC-TN (liquid) $40 per sample
Sample preparation (per sample)
Dionex ASE (solvent extraction) $90 per sample
Ashing $45
Sample Grinding and drying (~50g) $60
Fine milling for CHNS or O (< 1g) $30
Freeze drying (fit inside 50ml vial) $45
Nutrient analysis (per sample)
Gallery Discrete Analyser (NO3 and NH4) $35 per sample
Anion by IC (per sample)
Dionex 2100: F-, Cl-, NO3-, SO42-, Br- $50 per sample
Sample dilution prep including analysis IC $55 per sample
Integrion IC with Combustion for solids F-, Cl-, SO42- $120 per sample
Stable isotopes analysis
IRMS: N2O, N2, CO2 gas by Cryo-IRMS $35 per sample
IRMS: C and N in solids by EA-IRMS $40 per sample
2H and 18O by LWIA $40 per sample
Sample grining, drying and packing (~20g) $15 per sample
Gas analysis (per sample)
N2O, CH4, CO2 gas by GC $15 per sample
Sample dilution prep plus analysis GHG $20 per sample
H2, O2, CO, CO2, N2, N2O, NO by GC $20 per sample
Evaculated vial rental $1 per sample

Sample submission forms

For assistance with preparing, labelling and submitting samples, please contact us.

Genomics

Overview

We are equipped with the latest genomic technology to offer a broad range of services from sample extraction, preparation and analysis. Our experienced staff operate from both NATA -accreditation and PC2 laboratories, enabling us to accommodate a variety of samples, both clinical and research. We can provide support for all aspects of the genomics sample preparation and analysis workflow, including DNA/RNA extraction, library preparation, quality control, manual and automated sample processing, Next-Generation-Sequencing, Microarrays and bioinformatics analysis.

Our equipment is available to staff, students and external clients with both self-service and full-service options. Our experienced staff can provide training, advice and guidance on the experimental design process and the analysis of results, so that you achieve optimal outcomes.

Services

We routinely run a wide variety of services, that are continuously being upgraded as newer technologies emerge and listed below are the more commonly run services. We are also happy to work with researchers on protocol development for new methods or trialling unusual samples that may have not been tested with existing technologies.

Prices are largely dependent on sample number and will fluctuate accordingly. We are happy to help you plan your experiment so you can aim for sample numbers that give you the best value for money, whilst delivering statistically relevant results.

If you have any further enquiries in relation to the services we provide or need additional information, email carfgenomics@qut.edu.au.

Service  per sample/per project **
  Ideal sample number AUD/sample
DNA extraction - blood/saliva* 24 $33
DNA extraction - tissue*   Please enquire
DNA extraction - FFPE scrolls*   Please enquire
DNA/RNA co-extraction - FFPE scrolls*   Please enquire
DNA extraction (non-standard) Prices vary depending on sample type  
RNA extraction (non-standard) Prices vary depending on sample type  
FFPE extraction (non-standard) Prices vary depending on sample type  
Whole genome sequencing 5-60 **$6,976-$32,784
RNA sequencing 8-192 **$4,721-$68,801
DNA sequencing Prices vary depending on sample type  
Exome sequencing 32-570 **$14,059-$179,114
Clinical Exome sequencing* 24 Please enquire
Targeted and panel sequencing Prices vary depending on sample type  
Pan-Cancer panel sequencing 24 Please enquire
TSO500 HT DNA/RNA* 24 Please enquire
Hi-C sequencing Prices vary depending on sample type  
Sanger Sequencing   Please enquire
Single cell sequencing Prices vary depending on sample type  
Isoform sequencing 1-4 **$5,664-$7,752
Nanopore sequencing 1 (Multiplex) -24 **$12-$4,964
PacBio sequencing 1 (10-18kb) - 96 (16S) **$3,493-$4,128
Microbiome sequencing Prices vary depending on sample type  
Metagenomics Prices vary depending on sample type  
Epigenetics Prices vary depending on sample type  
Nanostring sequencing Prices vary depending on sample type  
ELISA assays Prices vary depending on sample type  
ChIP sequencing Prices vary depending on sample type  
MassARRAY SNP genotyping (GRC) 96 Please enquire
HRM Genotyping   Please enquire
RFLP Genotyping   Please enquire
Pyrosequencing SNP assay   Please enquire
Pyrosequencing CpG assay   Please enquire
SyBr Q-PCR   Please enquire
SNP microarray (global screening array) 364 **$110
SNP microarray (bisulfite modification and methylation EPIC array) 192 **$575
Targeted Ampliseq gene panel designing Arrange a consultation as early as possible for details  
Bioinformatics Arrange a consultation as early as possible for details  

* Optional NATA-accredited services

Technology and equipment

The equipment at our facilities has a range of applications and we are happy to discuss your needs to find what works best for you. Certain equipment is available for use by researchers with training available.

To find out more or discuss what you would like to do, email carfgenomics@qut.edu.au.

Quality control

Instrument Capabilities and features
Agilent Fragment Analyzer 5200 Uses automated parallel capillary electrophoresis to provide reliable and accurate quality control for DNA and RNA samples. Sample types include genomic DNA, NGS libraries, small RNA, cell free DNA, large DNA fragments and total RNA quality assessment.
Agilent 2100 Bioanalyzer The Agilent 2100 Bioanalyzer system is an established automated electrophoresis solution for the sample quality control of biomolecules for DNA, RNA, or protein analysis.
Agilent Femto pulse Powerful and automated pulsed-field capillary electrophoresis system. Easily achieve 10 times higher sensitivity for smears and up to 100 times for fragments. A meticulously designed optical detection platform and pulsed-field power enable unprecedented sensitivity, detecting nucleic acids into the lower femtogram range.
Tapestation 4200 This system is an established automated electrophoresis tool for DNA and RNA sample quality control. The flexible platform allows QC of a single sample, through to a full 96-well plate while maintaining cost-efficiency.
Qubit 4.0 This Fluorometer is the next generation of the popular benchtop fluorometer designed to accurately measure DNA, RNA, and protein quantity. The easy-to-use touch screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.
Qubit Flex A benchtop fluorometer designed for highly accurate quantification of DNA, RNA, microRNA, and protein. With the Qubit Flex Fluorometer you have the flexibility to directly measure fluorescence of up to eight samples simultaneously, which reduces assay variability.
Varioskan LUX microplate reader Designed for bioscience researchers with a wide variety of needs, this microplate reader comes equipped with a flexible range of measurement technologies including Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, and Time-Resolved Fluorescence. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation.
Nanodrop DNA, RNA and protein quantitation with only 1–2 µL of sample in seconds. Frequently used alongside Qubit to determine purity of samples.
Corbett Rotor Gene qPCR Instrument for quantitative real-time amplification that’s also capable of end-point analysis, nucleic acid concentration measurement and now HRM (high resolution melt).
Trinean Dropsense A compact, benchtop spectrophotometer for plate-based high-speed quantification of DNA, RNA or protein samples.

Preparation

Instrument Capabilities and features
Diagenode Bioruptor An ultrasonicator for the consistent and precise shearing of DNA, RNA and protein. Also has utility in cell lysis, liposome preparation and similar applications.
Nadia Instrument An automated, microfluidic droplet-based platform for single cell research that encapsulates up to 8 samples, in parallel, in under 20 mins. Over 50,000 single cells can be captured per cartridge in a run.
Nadia Innovate Enables the development of user-defined single cell protocols and applications. Newly developed protocols can be transferred to the Nadia Instrument for high throughput parallel operation.
QIAsymphony A fully automated extraction system that can accommodate up to 96 specimens in the one run. This bead-based technology enables purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials.
Covaris E220 A plate-based sonicator for reliable and reproducible fragmentation of samples in preparation for Next-Generation Sequencing library preparation.
Covaris M220 The M220 Focused-ultrasonicator offers the “Scientist’s Standard” in a compact, easy-to-use system making it the ideal DNA shearing solution for MiSeq and PGM users. AFA® technology in the M220 eliminates operator-induced variations, improves recoveries, increases efficiency, and provides standardized results.
Tecan liquid handling Innovative liquid handling technology that is flexible and reliable for high through-put SNP microarray processes.
Janus liquid handling A flexible automated liquid handling robot to meet specific application needs, including high-throughput nucleic acid normalisation, NGS sample preparation and sample pooling.
Eppendorf epMotion 507t Unique mixing performance with excellent temperature control to guarantee complete, dependable, and reproducible assay results.

Sequencing/analysis

Instrument Capabilities and features
ABI 3500 Genetic Analyzer This 8-capillary Genetic Analyzer is specifically configured with software for both fragment analysis and resequencing applications.
Agena MassARRAY System A MALDI-TOF mass spectrometry system utilising multiplex PCR to enable the detection of a variety of genetic variations, including SNPs, indels, translocations, copy number changes and methylation. Multiple variants in a sample can be queried simultaneously, based on multiplex PCR and probe compatibility.
Pacbio Revio High-throughput sequencing platform (up to 30 human genomes per run) delivering high-quality long-read sequencing data, making it ideal for a wide range of genomic applications, including de novo genome assembly, structural variant detection, and full-length transcriptome analysis.
Nanopore MinION, MK1C, Flongle and GridION A range of portable, self-contained sensing technology that uses nanopores embedded in high-tech electronics, to perform precise molecular analysis. Offers real-time data delivery of short to ultra-long fragments of native DNA or RNA.
Illumnia MiSeq Facilitates 0.3Gb to 15Gb of output with 1-25 million sequencing reads and 50 to 600 bp read lengths. Ideal for targeted re-sequencing, metagenomics, small genome sequencing and expression profiling sequencing projects.
Illumina NovaSeq 6000 Illumina’s most powerful sequencing system, designed for scalable throughput by offering output up to 6 Tb and 20 billion reads in dual flow cell mode. Flexible workflow with streamlined operations increasing efficiency at cost effective rates for HTP sampling.
Illumina NextSeq 1000 & 2000 The NextSeq 1000 and NextSeq 2000 Systems are thoughtfully designed to enable more insights on your benchtop. With 14 configurations and read lengths from 1 × 50bp to 2 × 300bp, these systems efficiently handle benchtop workflows while driving down costs and minimizing waste.
Ion GeneStudio™ S5 Plus The Ion GeneStudio S5 Plus System is a semiconductor-based next-generation sequencing (NGS) system that enables simple targeted sequencing workflows Ampliseq targeted gene sequencing, Ampliseq whole exome sequencing, Ampliseq Whole Transcriptome, RNASeq, small RNASeq: Human, mouse, rat samples.

BioRad Droplet Digital PCR

ddPCR technology enables high-throughput digital PCR in a manner that uses lower sample and reagent volumes while maintaining the sensitivity and precision that are the hallmarks of digital PCR. ddPCR technology provides an absolute count of target DNA copies per input sample without the need for running standard curves, making this technique ideal for measurements of target DNA, viral load analysis, and microbial quantification.
Nanostring nCounter Provides a simple and cost-effective solution for multiplex analysis of up to 800 RNA, DNA, or protein targets.  Accelerate your research with just 15 minutes total hands-on time without amplification, cDNA conversion, or library prep and generate publication ready figures in ~24 hours.
Nanostring GeoMx digital spatial profiler Combines the best of spatial and molecular profiling technologies by generating digital whole transcriptomes and profiling data for 100s of validated Protein analytes from up to 12 tissue slides per day. This unique combination of high-plex and high-throughput spatial profiling enables researchers to rapidly and quantitatively assess the biological implications of the heterogeneity within tissue samples.
Illumina iScan High-precision scanner that supports an extensive range of array applications. This system can scan thousands of array samples per day, without sacrificing data quality or reproducibility. It supports our expansive portfolio of genetic analysis assays, from high-throughput genotyping to DNA methylation analysis.
Dolomite Bio Nadia/Nadia Innovate A high-performance microfluidic platform designed for single-cell RNA sequencing (scRNA-seq). The addition of the Innovate module allows researchers to customize their single-cell workflows.

Using our services and equipment

We can provide support for all aspects of the genomics sample preparation and analysis workflow, including: DNA/RNA extraction library preparation quality control manual and automated sample processing bioinformatics analysis.

Our equipment is available to staff, students and external clients with both self-service and full-service options. Our experienced staff can provide training, advice and guidance on the experimental design process and the analysis of results, so that you achieve optimal outcomes.

If you need equipment not currently available in the CARF genomics laboratory, it is likely we can arrange access to this, either through our partner institutions or preferred service providers.

Diagnostic Sequencing

Operating under the supervision of Distinguished Professor Lyn Griffiths, ISO15189 NATA accredited diagnostic sequencing for neurological conditions is also available (NATA/RCPA accreditation number 14979). Conditions for which diagnostic sequencing is available include: Sporadic or Familial Hemiplegic Migraine (SHM or FHM), Episodic Ataxia type 1 and 2 (EA1, EA2), Spinocerebellar Ataxia type 6 (SCA6), Dravet Syndrome, Generalized Epilepsy with Febrile Seizures plus (GEFS+) Epileptic encephalopathy (some forms), Rapid-onset Dystonia-Parkinsonism (RDP), Alternating Hemiplegia of Childhood (ACH) Cerebellar Ataxia, Areflexia, Pes cavus, Optic atrophy, and Sensorineural hearing loss (CAPOS), Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL), Cerebral Autosomal Recessive Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CARASIL), COL4A1/COL4A2 leukoencephalopathy, Fabry disease, small vessel diseases and related neurological conditions.

Costs for all tests include sample processing and handling from blood, sequencing, variant interpretation and clinical report preparation. For details, please see: https://research.qut.edu.au/grc/diagnostic-testing/diagnostic-testing-pricing/

Test Type Capabilities and features
Directed Sequencing A Sanger sequencing method is used for targeted mutation testing of single exons for the conditions specified in our other tests. Most useful for confirmation of mutations already known to be present in a patient’s family. Cost: $220
Targeted Gene Panel Our Targeted Gene Panel uses Ion Torrent NGS technology to simultaneously interrogate fifteen genes that are implicated in neurogenetic disorders. Genes include: ATP1A2, SCN1A, SCN2A, TREX1, FOXC1, KCNK18, HTRA1, KCNA1, COL4A1, COL4A2, PRRT2, NOTCH3, CACNA1A, ATP1A3 and GLA. Cost: $580
Exome Sequencing Accredited whole exome sequencing using Ion Torrent NGS technology for the identification of neurogenetic disorders relating to our standard disorders. Cost: $1100

Histology

The Histology lab is a complete service laboratory located at our Kelvin Grove campus.

Our laboratory can process, embed, section, stain and image fresh or fixed tissue, and specialises in expert, reproducible, high throughput histological services. We offer:

  • routine paraffin, cryo and more specialised resin histology services
  • soft and hard tissue capacity, specialising in bone tissue histology
  • rapid decalcification of mineralised samples
  • client designed tissue micro array blocks
  • automated immunostaining (enzyme labels, fluorescence labels, multiplexing, in situ hybridisation, NanoString protocols)
  • specialised imaging capability including brightfield and fluorescence modalities for stereomicroscopy and light microscopy
  • high throughput slide scanning.

Sample preparation

We work with human, animal and plant samples often with implanted medical and regenerative devices. To ensure optimal sample preparation, we can:

  • rapidly decalcify hard or mineralised tissues before processing for routine paraffin histology and/or immunohistochemistry options
  • embed mineralised samples in resin and section (thin or ground) to better preserve tissue morphology (ideal for bone and metallic/ceramic implants common in dentistry and orthopaedics)
  • rapidly freeze and cryo section samples with labile antigens or processing-sensitive components prior to staining.

Accessing services and equipment

Our histology laboratory is available to staff and students and external clients at a competitive price. It provides both self-service and full-service tissue processing, sectioning, histochemical and immunohistochemical staining and automated, high throughput slide scanning.

Our experienced staff can provide training, advice and guidance on the experimental process and the analysis of results, so that you achieve optimal outcomes.

Booking instructions

To arrange a consultation, email histology@qut.edu.au

Conditions

  • Work will not commence until a quote has been agreed upon.
  • Machine use will be charged as 'per use' for any period less than an hour and in full 1-hour increments thereafter.
  • Paraffin sections will be placed onto slides with 2 sections per slide, sample size permitting.
  • If embedding is being undertaken, the client must be present to advise on the orientation of the sample.
  • Automated staining may be set up for specific stains.
  • A minimum of 10 slides is required for all paraffin sectioning and staining requests.

Indicative pricing for external researchers

Machine use
Machine Per hour
Exakt saw $45
Exakt grinder $45
TegraPol grinder/polisher $45
Resin microtome $45
Histology services

Prices include labour unless stated otherwise.

Service Labour per hour Per sample Per slide
Tissue processing Not applicable $5 Not applicable
Paraffin embedding $100 $6 Not applicable
Paraffin sectioning $100 Not applicable $7.50
Resin embedding - 25mL mould $100 $100 Not applicable
Resin embedding - 100mL mould $100 $400 Not applicable
Resin ground section - large $100 Not applicable $45
Resin ground section - small $100 Not applicable $30
Automatic H&E staining and coverslipping Not applicable Not applicable $7
Automatic counterstain (Hx after IHC) and coverslipping Not applicable Not applicable $4
Other simple stains and coverslipping - Safranin O, Oil Red O, Sirius Red, Toluidine Blue, von Kossa, Trichrome, Methyl Green Not applicable Not applicable $8 - $12
Slide scanning (20 slides minimum) Not applicable Not applicable $10
Training $95 Not applicable Not applicable

Indicative pricing for Commercal Service

Machine use
Machine Per hour
Exakt saw $60
Exakt grinder $60
TegraPol grinder/polisher $60
Resin microtome $60
Histology services

Prices include labour unless stated otherwise.

Service Labour per hour Per sample Per slide
Tissue processing Not applicable $8 Not applicable
Paraffin embedding $130 $8 Not applicable
Paraffin sectioning $130 Not applicable $10
Resin embedding - 25mL mould $130 $150 Not applicable
Resin embedding - 100mL mould $130 $600 Not applicable
Resin ground section - extra large $550 Not applicable $65
Resin ground section - small $425 Not applicable $45
Automatic H&E staining and coverslipping Not applicable Not applicable $10
Automatic counterstain (Hx after IHC) and coverslipping Not applicable Not applicable $8
Other simple stains and coverslipping - Safranin O, Oil Red O, Sirius Red, Toluidine Blue, von Kossa, Trichrome, Methyl Green Not applicable Not applicable $15 - $20
Slide scanning (20 slides minimum) Not applicable Not applicable $15
Training $130 Not applicable Not applicable

Equipment

Our state-of-the-art equipment allows for:

  • paraffin histology
  • resin histology
  • cryo histology
  • high throughput processing and slide scanning
  • advanced light microscopy
  • image analysis
  • customised tissue micro array blocks

Paraffin histology

Instrument Capabilities and features
Milestone KOS Microwave HistoSTATION Rapidly decalcify specimens prior to paraffin processing with customisable programs. Perform antigen retrieval, microwave assisted histochemical and immunohistochemical staining.
Tissue Processor Automatic tissue processor with multiple, customisable programs capable of handling standard, mega and super mega cassettes.
Embedding Station Embed paraffin infiltrated soft and decalcified tissue samples prior to sectioning
Leica Rotary Microtomes (RM 2135, 2235, 2245, 2265) Manual and semi-automated microtomes for preparing histological sections (2µm+ thickness) from paraffin or resin embedded tissue.
Tissue MicroArrayer system (Unitma Quick-Ray UT06) Manual, customisable production of tissue microarray blocks, which can consolidate 10 to 100 target samples into a single paraffin block. Applies to cancer studies requiring high throughput screening or comparison, production of quality control blocks and miniaturisation of small or difficult to source tissues. Can provide simultaneous analysis of pathology tissue samples, providing cost and time savings for reagents and histochemical, immunohistochemical, immunofluorescence and in situ hybridisation techniques.
Cassette Writer Reliable, automated, permanent, highly legible labelling of biomedical histology cassettes. The bar code functionality integrates well with most laboratory information management systems.
Slide Printer Reliable, automated, permanent, highly legible labelling of microscopy slides used in biomedical, plant or material sciences fields. The bar code functionality integrates well with most laboratory information management systems.

Resin histology

Instrument Capabilities and features
Resin infiltration and embedding Embed hard and mineralised samples (with or without polymeric, metallic or ceramic implants) in a resin matrix prior to sectioning and histochemical and immunohistochemical staining.
EXAKT Precision Diamond Bandsaw Gross dissection of fresh or fixed calcified tissues and other hard samples.
EXAKT Precision Micro Grinder Prepare ground 30-50µm sections of resin embedded samples for staining and analysis.
Reichert-Jung Sledge Microtomes (Polycut D6907) Semi-automated microtomes for preparing histological sections (10µm+ thickness) from large paraffin embedded tissues or hard mineralised samples +/- scaffolds, medical or regenerative devices.

Cryo histology

Instrument Capabilities and features
Thermofisher Scientific Cryostar NX70 Prepare frozen sections for staining from fresh tissue by sectioning at <-10°C temperatures. Achieve results on fresh tissue without the need for fixation or processing. Suitable for immunohistochemical studies on labile antigens.

High throughput staining

Instrument Capabilities and features
Leica High Throughput Autostainer XL An automated staining machine with multiple staining protocols. Simultaneous staining provides high reproducability both within and between staining runs.
Leica Robotic Coverslipper Automates the application of coverslips neatly and accurately, optimising slide longevity and scanning results.
Leica Bond RX Immunostainer Fully automated, open platform, highly customisable research stainer. Capabilities include immunohistochemical (IHC), immunofluorescence (IF) and in situ hybridisation (ISH; FISH;CISH) techniques, tyramide signal amplification, microRNA, upstream NanoString protocols and, single or multi-plex staining, Simultaneous staining of up to 30 slides with different protocols.

Imaging and image analysis

Instrument Capabilities and features
3D Histech Slide Scanner Acquisition of high quality digital images at up to x70 magnification with additional digital zoom. Provision of automated scanning in brightfield and fluorescence modes with barcoding, z-stacking and extended depth of focus functionalities.  Access tailor made, application-specific resolution analysis packages for commercially produced or in-house generated tissue micro-arrays. Excellent for desktop analysis presentations and publications. Ideally suited for digital archiving of valuable, irreplaceable and/or fragile slide libraries for research and industry. Provides increased accessibility to samples by authorised stakeholders in local and remote locations.
Nikon MacroImager Image capture and annotation for large samples.
Nikon SMZ25 Fluorescence Stereomicroscope Brightfield and fluorescence (4 channels) microscopic evaluation of large, gross or high profile samples with digital camera recording. Capable of extended depth of field and time lapse. Can image human, animal, plant and synthesised materials on supports or in tissue culture plates/wells.
Nikon SMZ745T Stereomicroscope on boom stand Brightfield microscopic evaluation and quality control assessment of large, gross or high profile samples with digital camera recording. Can image human, animal and plant samples, cultured cells in wells and synthesised materials including scaffolds, hydrogels and medical devices.
Osteomeasure Histomorphometry Suite Automatically calculate measurements of over 175 parameters of bone and tissue sections based on ASBMR Standard Nomenclature using imported images.
Zeiss AxioImager M2 upright microscope Brightfield, darkfield and fluorescence (6 channels) microscopic evaluation of thin sections with digital camera recording. Motorised X, Y and Z stage permits tiling of large samples with excellent stitching of image sets.
Zeiss AxioLab A1 upright microscope Brightfield microscopic evlauation and quality control assessment of thin samples including scaffolds and sections with digital camera recording capability.

Magnetic resonance spectroscopy

Magnetic Resonance referes to a number of techniques that allows us to investigate materials at the molecular scale.

Overview

Our nuclear magnetic resonance and electron paramagnetic resonance spectrometers allow us to investigate the structure, dynamics and interactions of materials at the molecular scale.

The Magnetic Resonance lab hosts several Nuclear Magnetic Resonance (NMR) spectrometers and an Electron Paramagnetic Resonance (EPR) spectrometer.

Equipment

The laboratory specialises in two different modalities of magnetic resonance spectroscopy:

  • Nuclear Magnetic Resonance (NMR) spectroscopy for the study of a range of nuclei (typically 1H, 13C, 19F, 31P) in solution-state samples
  • Electron Paramagnetic Resonance (EPR) spectroscopy for the study of molecular species which contain one or more unpaired electrons, including stable free radicals, antioxidants, transition metal ions and reactive intermediates.

Nuclear Magnetic Resonance (NMR) spectrometers

Instrument Capabilities and features
Bruker Avance IIIHD 600 MHz

High-resolution NMR spectrometer typically configured for automated 1D and 2D spectroscopy of solution state samples under ambient conditions.

Probe: 5mm broadband BBO probe (1H and 107Ag to 19F) with 2H lock channel and Z gradient. Temperature range -150 to +150°C

SampleJet sample changer operating under IconNMR automation. BCU-I cooling unit for temperature control.

Bruker Avance IIIHD 400 MHz

High-resolution NMR spectrometer with; BBO probes for 1D and 2D spectroscopy of solution state samples under ambient or variable-temperature conditions; High-Resolution Magic Angle Spinning (HR-MAS) probe for spectroscopy of gel-state samples (e.g. swollen polymers, swollen beads, tissue samples).

  • Probe: 5mm broadband BBO probe (1H and 107Ag to 19F) with 2H lock channel and Z gradient. Temperature range -150 to +150°C
  • Probe: HR-MAS probe (1H, 13C) with 2H lock channel. Temperature range -20 to +80°C

Liquid nitrogen dewar and boil-off heater for low-temperature measurements.

Bruker Avance Neo 400 MHz

High-resolution NMR spectrometer with; TBO probe for 1D and 2D spectroscopy of solution state samples under ambient or variable-temperature conditions; High-Resolution CP Magic Angle Spinning (CP-MAS) probe for spectroscopy of solid-state samples.

  • Probe: 5mm broadband TBO probe (1H, 19F and 31P to 107Ag) with 2H lock channel and Z gradient. Temperature range -150 to +150°C
  • Probe: 4 mm broadband CP-MAS probe (1H, 19F and 31P to 15N) with 15 kHz rotational frequency. Temperature range -80 to +200°C

SampleCase sample changer operating under IconNMR automation. BCU-II cooling unit for temperature control.

Bruker Avance Neo 400 MHz

High-resolution NMR spectrometer with; BBFO probe for 1D and 2D spectroscopy of solution state samples under ambient or variable-temperature conditions; QUAD Systems photoprobe for in-site irradiation of solution state saples.

  • Probe: 5mm broadband BBFO probe (1H, 31P to 107Ag) with 2H lock channel and Z gradient. Temperature range -150 to +150°C
  • Probe: 5mm 2-channel probe (1H, 13C) with 2H lock channel and Z gradient, optimised for laser irradiation of sample via fibre optic. Temperature range 0 to 50°C

SampleCase sample changer operating under IconNMR automation. BCU-II cooling unit for temperature control.

Electron Paramagnetic Resonance (EPR) spectrometer

Instrument Capabilities and features
Bruker Magnettech ESR5000 Benchtop EPR spectrometer with variety of sample tube holders, from capillary up to 5mm tubes. Variable temperature measurements -170 to +200°C. Access port and accessories for in-situ sample irradiation.

Using our services and equipment

Our equipment and services are available to QUT staff and students, external researchers and commercial clients.

The Magnetic Resonance facility typically operates under a self-service (unassisted) model for trained users, with some full-service (with technical assistance) options as listed below. Our experienced staff can provide training, advice and guidance on the experimental process and the analysis of results, so that you achieve optimal outcomes.

Due to the complex nature of most Magnetic Resonance experiments, we will work with you to assess the feasibility of the analysis, timeframes and costs for your project.

Indicative pricing for assisted use

Prices are indicative and vary depending on whether work is for external researchers or commercial organisations, and who carries out the work. We will provide a costing proposal for your approval before any work starts. Bookings are limited to 2 hours during the day (8am - 5pm).

Equipment, service or analysis External researchers Commercial organisations
NMR or EPR spectroscopy (per hour) $210 $515
NMR or EPR instrument training (per hour) $120 $285
Assistance with sample preparation, method development, data analysis, report preparation (per hour) $120 $285

Microscopy

We prepare specimens and carry out both optical microscopy and electron microscopy.

Overview

Our microscopy laboratory has a suite of routine and advanced light microscopes and electron microscopes that enable users to conduct innovative research. We also offer geological sample preparation, and nanoscale imaging including ion beam microscopy.

Equipment

Our state-of-the-art equipment allows for optical microscopy, electron microscopy and specimen preparation.

Optical microscopy

Our optical microscopy instruments include stereo, petrographic, metallographic, fluorescence, and confocal microscopes. The laboratory can carry out light microscopy and thin film characterisation.

Light microscopy
Instrument Capabilities and features
Leica M125 Zoom Stereo Microscope Image stack collection and extended focus reconstruction. LED ringlight, gooseneck lights and motorised z-stand. Also has transmitted brightfield, darkfield, and oblique illumination.
Nikon Eclipse LV100ND Petrographic Microscope Polarised light optics in both transmission and reflection, rotating stage and motorised z-drive for 3D and extended depth of focus imaging. Compensator and full and quarter wave plates available for additional analysis.
Nikon Eclipse Ni Upright microscope with transmitted brightfield and DIC. Motorised nosepiece and condenser for ease of use.
Leica DMi8A Inverted Microscope Inverted materials microscope for metallography in brightfield and polarised light including scanning stage and manual focus drive.
Evident FluoView4000 Confocal Microscope (Upright) Confocal imaging optimised for fixed biological samples and materials samples. Fast galvanometric and resonant scanners. 405, 488 561 and 640 nm lasers. Tuneable SilVIR spectral detectors. Reflected light imaging available.
Nikon Eclipse Ti Inverted Microscope Imaging of fluorescent or transparent samples. Equipped with fluorescent filters for DAPI, FITC, TRITC, and Cy5 and similar fluorescent labels. Also equipped with transmitted brightfield, DIC, and polarising optics. Motorised stage with stage inserts for imaging large areas on single slides, or multi-well plates.

Electron microscopy

Our electron microscopy instruments include an electron probe microanalyser (FE-EPMA), focused ion beam (FIB) microscopes, scanning electron microscopes (SEMs) and transmission electron microscopes (TEMs).

Capabilities

We can:

  • embed, section, and stain fixed biological tissues or samples (SEM, TEM)
  • mount and polish materials (eg. minerals, glasses, metal alloys) in thin section, epoxy resin or conductive resin (SEM, TEM, EPMA)
  • perform microstructural and compositional analysis (TEM, SEM) (eg. electron diffraction, EDX on SEM and TEM)
  • produce high resolution TEM images (lattice structure information)
  • undertake tomography and Z-contrast imaging
  • perform nanostructuring (FIB) and HRTEM lamella preparation, targeted cross-sectioning, tomography, including running simulations (SRIM, FIB/SEM)
  • perform electron back-scattered diffraction (EBSD) to investigate structure and orientation of polished crystalline materials
  • use wavelength dispersive x-ray spectroscopy to generate high precision standards-based quantitative elemental analysis of solid materials (EPMA)
  • integrate panchromatic and hyperspectral cathodoluminescence imaging (UV- vis-near IR) into EPMA
  • carry out liberated mineral analysis (LMA)
  • carry out electron and ion (He, Ne) beam lithography for nanostructuring.
Electron probe microanalyser (EPMA)
Instrument Capabilities and features
JEOL JXA 8530F Hyperprobe

Field emission electron microprobe for high-precision, standards-based quantitative elemental analysis (Be to U) and x-ray mapping using Probe for EPMA and Probe Image software.

Electron gun operates up to 30 kV and several mA beam current, with detection limits routinely ≤100 ppm and reaching <10 ppm.

Twelve analyzing crystals are distributed among five wavelength dispersive spectrometers (WDS) including a large crystal "L" type and two high count rate "H" types.

Detector array features two sealed Xe and three gas-flow proportional counters.

Additional capabilities for imaging and microanalysis include a Thermo Scientific EDS system with Pathfinder Pinnacle software, JEOL panchromatic cathodoluminescence (CL) detector, and both standard and high-resolution xCLent spectroscopic CL detectors and xCLent V software. Anti-contamination cold finger is available.

Scanning electron microscopes (SEMs)
Instrument Capabilities and features
Zeiss Sigma VP Field Emission Scanning Electron Microscope Zeiss Gemini column gives excellent imaging performance across the beam energy range for imaging delicate structures and surface features. Variable Pressure (VP) operation for insulating samples. Oxford XMax 50 Silicon Drift (SDD) EDS detector for rapid X-ray mapping and accurate analysis.
JEOL 7001F Scanning Electron Microscope A very stable instrument capable of delivering very high beam currents in a small spot size for X-ray and electron backscatter diffraction (EBSD) analysis. Automated feature detection and analysis software. Field emission gun, Oxford XMax 80 X-ray detector, Inca gunshot residue (GSR), Oxford EBSD system.
Hitachi SU7000 Scanning Electron Microscope A high-resolution imaging SEM with stage biasing for low-voltage imaging of delicate structures and surface features and variable pressure mode for insulating samples. Equipped with dual in-column detectors, a chamber SE detector, and a 5-segment retractable BSE detector, offering multi-signal and simultaneous display. Fitted with Oxford Ultim Max 100 EDS detector for rapid X-ray mapping and accurate microanalysis. Aztec GSR gunshot residue software package is included as well.
TESCAN Mira3 Scanning Electron Microscope Stage biasing for low-voltage imaging of delicate structures and surface features. Variable Pressure (VP) mode for insulating samples available with a cold stage. Fitted with a 60 mm² Thermo Ultra Dry EDS Detector. The microscope has an Electron Beam Lithography package for nanostructuring.
TESCAN TIMA Field Emission Scanning Electron Microscope An automated mineral analysis system that fully integrates backscattered electron (BSE) and cathodoluminescence (CL) imaging with energy dispersive X-ray (EDX) spectrometry for fast quantitative analysis of rock samples and natural resource materials such as ores, tailings and smelter products.
Phenom XL G2 Desktop Scanning Electron Microscope Contains a large sample holder with motorised stage navigation, low/medium/high vacuum modes, light optical and electron optical (BSE and SE) modes. The Phenom includes an EDS detector with integrated software. The resolution of this microscope is excellent due to the thermionic (CeB6) source (<10 nm).
Focused ion beam instruments (FIB and HIM)
Instrument Capabilities and features
Zeiss ORION NanoFab Helium Ion Microscope (HIM) High-resolution scanning ion microscope that employs high energy ion beam (helium or neon) for sub-nanometer imaging and nanofabrication. HIM is equipped with electron flood gun for charge compensation and imaging of nonconductive materials. The use of dual-ion beams (such as helium and neon) allows for a wide range of applications, including nanoscale ion implantation and defect engineering.
TESCAN S8000x Xe Plasma FIB-SEM FIB-SEM instrument that combines Xenon plasma FIB with field-free UHR FE-SEM, for characterization of materials in large (up to 0.5 mm in width) FIB cross sections, multi-modal FIB-SEM tomography for 3D reconstruction (including 3D EDS and EBSD analysis) and fabricating micro- and nanostructures, using the implantation-free Xe ion beam (e.g. TEM lamellas and micropillars for mechanical testing).
Transmission electron microscopes (TEMs)
Instrument Capabilities and features
JEOL 2100 200 kV Transmission Electron Microscope High-resolution imaging capabilities for a range of materials and nanotechnology applications. High-sensitivity silicon drift X-ray detector (Oxford XMax) for compositional analysis. Ultra-high-resolution pole piece, TVIPS Fx416 4K CCD camera, LaB6 gun.
JEOL 1400 120 kV Transmission Electron Microscope High-contrast optics, optimised for imaging of biological samples, and high tilt range for acquisition of tomographic datasets. TVIPS F4216 2K CCD camera, tomography holder, cryo holder, tungsten gun.
JEOL ARM200F "NeoARM" Aberration Corrected Transmission Electron Microscope. Capable of sub-angstrom imaging and sub-nanometre compositional analysis of a wide range of samples. High resolution pole piece, capable of supporting high tilt range for tomography experiments. Equipped with Gatan OneView camera and Cold Field Emission Gun.
Sample preparation
Instrument Capabilities and features
Gatan Cryoplunge 3 Cryofixation of biological samples for transmission electron microscopy.
Pelco Biowave Pro Microwave Tissue Processor Microwave accelerated processing of tissue samples for scanning electron microscopy and transmission electron microscopy.
Leica EM UC6 Ultramicrotome Sample preparation of embedded biological samples for transmission electron microscope characterisation.
Leica EM UC7 Ultramicrotome Sample preparation of embedded biological samples for transmission electron microscope characterisation.
Leica EM TXP Target preparation device for milling, sawing, grinding and polishing samples.
Gatan Model 691 Precision Ion Polishing System (PIPS) Precise ion milling of samples for transmission electron microscopy.
Gatan Model 682 Precision Etching and Coating Systems (PECS) Ion milling of light microscope and scanning electron microscope samples up to 25 mm diameter mounted blocks. High quality sputtered coatings of chromium and carbon.
Leica EM-SCD005 Sputter Coater Gold sputter coating.
Leica EM ACE600 High Vacuum Coaters Two ACE600 coaters - one for platinum sputter coating and another with dual heads for carbon thread coating or iridium sputter coating.
Safematic CCU-010 HV High Vacuum coater Carbon thread coating.
Tousimis Autosamdri-815 Critical Point Drier (CPD) Automated CPD for dehydration of samples for electron microscopy.
Evactron 25 Decontaminator with Softclean Chamber Gentle removal of hydrocarbon contamination from samples and components by oxygen free-radical stream. Can also be used to glow discharge sample surfaces for a hydrophilic finish to improve fluid mounting.
E.A. Fischione Model 2000 Specimen Preparation System Precision grinding of samples for transmission electron microscopy.
E.A. Fischione Model 330 Ultrasonic Disk Cutter Cutting undamaged 3mm disk samples for transmission electron microscopy.
Fischione Model 110Twin Jet Electropolisher Thinning 3mm metal samples for transmission electron microscope (TEM) imaging.

Using our services and equipment

Our microscopy equipment is available to staff, students, external researchers and commercial organisations at a competitive price.The laboratory provides both self-service and full-service options, with comprehensive instrument training available for internal and external academic researchers as well as commercial clients. Our experienced staff can provide advice and guidance on the experimental procedures and design and the analysis of results in order to achieve optimal outcomes.

Indicative pricing

Prices are indicative and vary depending on whether work is for external researchers or commercial organisations, and who carries out the work. We will provide a costing proposal for your approval before any work starts.

Indicative pricing for external researchers
Equipment, service or analysis With technician assistanceUnassisted
SEM microscopy (per hour)
JEOL 7001F $170 $80
Hitachi SU7000 $170 $80
Zeiss Sigma $170 $80
TESCAN Mira 3 $170 $80
TESCAN TIMA $170 $80
Phenom XL G2 $170 $80
Electron probe microanalysis (per hour)
JEOL 8530F $170 $80
TEM microscopy (per hour)
JEOL 1400 $170 $80
JEOL 2100 $170 $80
JEOL ARM200F "NEOARM" $170 $80
Interpretation and reporting $105 N/A
Ion microscopy (per hour)
Helium Ion Microscopy $230 $120
Xe Plasma FIB $230 $120
Interpretation and reporting $105 N/A
Other microscopy (per hour)
Light microscopes $145 $55
Nikon A1R Confocal $170 $80
Other services (per hour)
Microscopy preparation $105 N/A
Consultation and report writing $105 N/A
Indicative pricing for commercial organisations
Equipment, service or analysis With technician assistanceUnassisted
SEM microscopy (per hour)
JEOL 7001F $430 $295
Hitachi SU7000 $430 $295
Zeiss Sigma $430 $295
TESCAN Mira 3 $430 $295
TESCAN TIMA $430 $295
Phenom XL G2 $430 $295
Electron probe microanalysis (per hour)
JEOL 8530F $430 N/A
TEM microscopy (per hour)
JEOL 1400 $430 N/A
JEOL 2100 $430 N/A
JEOL ARM200F "NEOARM" $430 N/A
Ion microscopy (per hour)
Helium Ion Microscopy $605 N/A
Xe Plasma FIB $605 N/A
Interpretation and reporting $265 -N/A
Other microscopy (per hour)
Light microscopes $245 $110
Nikon A1R Confocal $325 $190
Other services (per hour)
Microscopy preparation $185 N/A
Materials preparation $185 N/A
Consultion and report writing $270 N/A

Molecular mass spectrometry

We offer state-of-the-art spectrometry platforms that allow for qualitative, quantitative and structural analysis.

Overview

Our laboratory houses cutting-edge mass spectrometry instrumentation interfaced with liquid chromatography and gas chromatography capabilities. This equipment enables selective identification and reproducible quantification of trace-level molecules in complex samples.

Equipment

Our state-of-the-art equipment allows for:

  • liquid chromatography mass spectrometry (LC-MS)
  • gas chromatography mass spectrometry (GC-MS)
  • sample preparation.

Liquid chromatography mass spectrometry (LC-MS)

Instrument Capabilities and features
Shimadzu Triple Quadrupole 8050 LC-MS/MS High-sensitivity and ultra-fast polarity switching platform with ESI and APCI ionisation capabilities.
Chromatography: Nexera UPLC.
Application: Targeted quantitation of trace-level compounds (eg. nucleotides, pharmaceuticals, metabolites and lipids).
SCIEX Triple Quadrupole Trap 6500 LC-MS/MS platform High-sensitivity platform for targeted, quantitative MRM-based assays. Also features differential ion mobility (DMS) capabilities for enhanced separation/structural elucidation of isobars and epimers.
Chromatography: Prominence HPLC.
Application: Shotgun lipidomics, quantitation of peptides.
Thermo Fisher LTQ Orbitrap Elite High-resolution MS/MS platform equipped with an automated nanospray robot. 
Chromatography: Dionex UPLC.
Application: Untargeted discovery of synthetic molecules and endogenous metabolites.
Thermo Fisher LTQ-XL Linear ion trap MS/MS platform with photodissociation capabilities.
Chromatography: Dionex UPLC.
Application: Low resolution identification of synthetic molecules.
Waters Synapt G2-Si Ion-mobility enabled quadrupole time of flight platform, equipped with LC-ESI or Desorption Electrospray Ionisation (DESI) ion sources.
Chromatography: Waters Acquity i-Class UPLC.
Application: Mass spectrometry imaging via DESI, ion-mobility MS.
Waters Select Series Cyclic IMS Ultra-high resolution ion mobility platform for unparalleled resolution of isobars and isomers. Features ESI and DESI ion sources.
Chromatography: Waters Acquity Premier UPLC.
Application: Ion mobility separation of isomeric molecules of synthetic or biological origin.

Gas chromatography mass spectrometry (GC-MS)

Instrument Capabilities and features
Shimadzu Triple Quadrupole 8040 GC-MS/MS Capable of high sensitivity, highly reproducible, MRM-based quantitation of trace-level compounds. Equipped with high-efficiency ion source.
Auto-sampler: liquid injection, SPME, headspace, automated derivatization.
Application: Metabolite profiling with online derivatization.
Shimadzu Triple Quadrupole 8050 GC-MS/MS Capable of quantitative analyses of ultra-trace level compounds, down to the femtogram level. Equipped with high efficiency detector.
Auto-sampler: liquid injection, SPME, headspace, automated derivatization.
Application: Quantitative analysis of environmental pollutants, volatiles, residual solvents and metabolites.
Shimadzu QP2020 with Pyrolyzer Multi-functional pyrolyzer with vertical micro-furnace enables analysis of almost all sample forms.
Application: Analysis of insoluble materials and compounds, trace level polymer materials without pre-treatment.
Shimadzu QP2020 NX with Thermal Desorber Equipped with TD-30 unit. Application: Analysis of VOCs collected in thermal desorption tubes.

Sample preparation

Instrument Capabilities and features
Benchtop preparation Includes ultra-high purity solvents and chemicals, derivatisation reagents, shakers, incubators and temperature-controlled centrifuges.
Eppendorf Thermomixer Programmable heating, cooling and mixing. Adapters for various tube and plate formats available.
Perkin Elmer JANUS G3 MDT Automated Workstation Liquid handling system for automated sample preparation.

Using our services and equipment

Our equipment and services are available to QUT staff and students, external researchers and commercial clients with both self-service (unassisted) and full-service (with technical assistance) options.

Due to the complex nature of most mass spectrometry experiments, please email carfproteomics@qut.edu.au to discuss your project requirements.

All prices are ex GST.

Indicative pricing

Liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS)
Service description External researchers/Collaborative Commercial organisations
Analysis using established methods
Data acquisition, analysis and report $275 first sample
$85 each additional sample
$685 first sample
$200 each additional sample
Sample preparation $440 first sample
$30 each additional sample
$1,100 first sample
$75 each additional sample
Analysis requiring method development
Pilot acquisition, analysis and report from $5,500 from $13,750
Sample preparation (pilot investigation) from $2,200 from $4,950
Method validation by agreement by agreement

Terms and conditions

  • Testing Services agreement is required.
  • Pricing above applies to assisted use only.
  • Method validation against client requirements.
  • Additional costs might include chromatography columns, analytical standards and other specialist consumables.

Hourly access fee

Liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS)
Service description External researchers Commercial organisations
Access $100/hour N/A
Training $210/hour N/A

Annual access fee (facility subscription)

Liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS)
Service description External researchers Commercial organisations
Access and training $6,950 $16,950

Terms and conditions

  • The training fee is for unassisted use, and does not cover assistance in sample preparation, method development and data interpretation. A separate arrangement must be made for these services.
  • Requires CARF induction and Work in CARF agreement.
  • Fee covers access to the mass spectrometry laboratory for a single user and up to 300 hours of instrument time which are to be used within 12 months from completing CARF induction.
  • Includes 2 days of instrument training and ongoing consultation with CARF team during the subscription period.
  • Subscriptions are paid in advance. Unused hours cannot be transferred, extended, or reimbursed.
  • Users are responsible for booking instrumentation, data acquisition and interpretation, and purchasing project-specific consumables (e.g., autosampler vials, chromatography columns and analytical standards).

Physical and mechanical properties

We analyse the physical and mechanical properties of any item, including size, porosity, surface area and thermal qualities.

Overview

At our laboratory, we specialize in analyzing the physical and mechanical attributes of various samples. Researchers have the opportunity to thoroughly characterize materials in terms of:

  • Mechanical properties, encompassing hardness and tensile strength
  • Surface area, porosity and particle size
  • Thermal properties, with the capability to measure changes up to 1600°C.
  • Thermoelectric properties.

We provide comprehensive analysis services, which include sample preparation and detailed formal reporting. Additionally, we offer training and ongoing support for clients who wish to conduct their own measurements. Our flexible approach allows us to tailor arrangements to meet the specific needs of our customers.

Mechanical properties characterisation

Instrument Capabilities and features
Hysitron TI 950 TriboIndentor Nanomechanical characterisation and testing instrument, with dual-head testing capability for nano/micro scale connectivity.
Anton Paar MCR302 Rheometer Flow properties of low to high viscosity materials in a temperature range between 30°C to 200°C.
TA Discovery DMA 850 Dynamic Mechanical Analyser Viscoelastic properties of solid materials in a temperature range of -85°C to 600°C and relative fumidity up to 95%.
Struers Duramin-40 AC3 hardness tester Performs Vickers and Knoop hardness tests in a force range from 10 g to 62.5 kg.
Tytron 250 Microforce Testing System (MTS) Low-load tensile test machine rated up to 250 N. Capable of standard and fatigue testing and three-point flexural testing. Available load cells are 250 N and 10 N.

Surface area, porosity and particle analysis

Instrument Capabilities and features
Malvern Mastersizer 3000 Particle size measurements for suspended particles 1mm to 0.1 micron.
Malvern Zetasizer Nano ZS Particle size and zeta potential for suspended particles <1000 nm.
Micromeritics 3-Flex Surface and Porosity Analyzer Surface area (BET), pore size distribution (<800 angstroms) of solid materials using gas absorption/desorption. Nitrogen, argon carbon dioxide, hydrogen, oxygen. Best for micropores.
Micromeritics ASAP 2020 Surface Area and Porosity Analyzer Surface area (BET), pore size distribution (<800 angstroms) of solid materials using gas absorption/desorption. Nitrogen, argon carbon dioxide. Best for micropores. Chemisorption also available.
Micromeritics Tristar II 3020 Surface Area and Porosity Analyzer Surface area (BET), pore size distribution (<800 angstroms) of solid materials using nitrogen or argon. Three measurement ports. Best for mesoporous materials.
Anton Paar Ultrapyc He Pycnometer True solid density with helium gas entering connected pores as small as 0.2 nm.

Thermal analysis

Instrument Capabilities and features
Netzsch 402C Dilatometer A vacuum-tight pushrod dilatometer for determining linear thermal length expansion in solids and powders up to 2000°C.
Netzsch STA 449F3 Jupiter Simultaneous Thermal Analyser Simultaneous thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Measurement of mass changes and thermally induced phase changes between 25°C and 1650°C. GCMS and gas phase FTIR analysis of evolved gases are available.
Netzsch LFA 467 Hyperflash Thermal diffusivity and conductivity, and specific heat capacity measurements in the temperature range of room temperature to 500°C.
Netzsch HFM 436 Lambda Heatflow meter For thermal conductivity measurements on insulating materials. Temperature range 30°C to 50°C.
Netzsch Phoenix DSC For differential scanning calorimetry (DSC) measurement of samples between -90 °C and 400 °C. DSC during optical curing can be measured on this instrument.

Thermoelectrics

Instrument Capabilities and features
Ulvac ZEM3 Seebek coefficient Measures Seebek coefficient of thermoelectric materials.
Ulvac PEM2 Measures power efficiency of thermoelectric materials.
Advance Riko STPM Scanning thermal probe microscopy.
Advance Riko Laser PIT-M2 Thermal diffusivity of thin films.

Sample preparation

Instrument Capabilities and features
Micromeritics Smart Vac Prep Sample preparation for surface area/porosity analysis on Micromeritics 3-Flex.
Micromeritics VacPrep 061 Degasser Sample preparation for surface area and porosity analysis through gas absorption/desorption, with vacuum mode that prepares samples by heating and evacuation. For samples run on Tristar II.
Sample splitters Riffle splitter for representative dry sample preparation. Funnel splitter for representative sampling from samples in aqueous suspension.
Sieves 1.5 mm and 2.4 mm sieves for preparation of samples for Mastersizer.
Plasma cleaner Diener plasma cleaner for cleaning contamination from sample surfaces.
Branson Probe sonicator Probe sonicator fixed with micro-tip. Suitable for sample volumes up to 5 mL.

Using our services and equipment

Our equipment is available to staff, students, external researchers, and commercial organisations at a competitive price. Our laboratory provides both self-service and full-service options.

Our experienced staff can provide training, advice and guidance on the experimental process and the analysis of results, so that you achieve optimal outcomes.

Indicative pricing

Prices are indicative and vary depending on whether work is for external researchers or commercial organisations, and who carries out the work. We will provide a costing proposal for your approval before any work starts.

Indicative pricing for external researchers (per hour)

CARF technician assistance for the equipment incurs an additional $100 per hour for external researchers, which is indicated in the costs below. External researchers wishing to operate the instruments unassisted must attend initial training which will incur training costs. Training costs are calculated as the instrument hire rates plus staff rates for the duration of the training.

Equipment, service or analysis with technician assistance per hour Unassisted instrument hire per hour
Nanoindentation $210 (min 4 hours) $620 per day
Rheology studies $190 $90
Tehsile testing (max 250N) $165 $65
Micron range particle sizing (Mastersizer) $165 $65
Nanometre range particle sizing / zeta potential - Malvern ZetaSizer $165 $65
Microhardness testing - Vickers $165 $65
Density by He pycnometry $165 $65
Data analysis and formal reporting $100 NA
Indicative pricing for external researchers (per sample)

Individuals wishing to operate the instruments unassisted must attend initial training which will incur training costs.

Please contact us for a training quote.

Equipment, service or analysis Run by staff (per sample) Run by client (per sample)
Thermal Analysis (TGA and DSC) up to 1000 C $280 $125
Thermal Analysis (TGA and DSC) above 1000 C $385 $175
FTIR gas analysis (coupled to TGA/DSC) $150 NA
Specific heat capacity $400 NA
Thermal conductivity by laser flash (includes specific heat capacity) $525 NA
Coefficient of thermal expansion, Tg, sintering temperature by dilatometry $175 $75
Low temperature DSC: - 90 to 500 ℃ $125 $75
Micron range particle sizing ( Mastersizer, incl SEM image, minimum 4 samples) $100 NA
Micron range particle sizing ( Mastersizer, no SEM image, minimum 4 samples) $60 NA
Nanometre range particle sizing (incl SEM image, minimum 4 samples) $100 NA
Nanometre range particle sizing / zeta potential (no SEM image, minimum 4 samples) $60 NA
Gas adsorption analysers (per batch of three samples) $455 (batch of 3 samples) $375 per day
Gas adsorption analysers (per sample) $175 NA
Density determination by He pycnometry $100 NA
Indicative pricing for commercial organisations

CARF technician assistance for the equipment incurs an additional $265 per hour for commercial organisations, which is indicated in the costs below. Organisations wishing to operate the instruments unassisted must attend initial training which will incur training costs.

Equipment, service or analysis With technician assistance Unassisted instrument hire, per hour
Particle analysis
Particle size distribution (laser diffraction) - no SEM image $105 per sample $135
Particle size distribution (laser diffraction) - including SEM image (meets ISO 13320:2009E) $220 per sample N/A
Particle size distribution (nano particles) - no SEM image $105 per sample $135
Particle size distribution (nano particles) - including SEM image (meets ISO 13320:2009E) $220 per sample N/A
Zeta potential (nano particles) $160 per sample $135
Density determination using He pycnometry (min 4 hours) $315 per hour $135
Specific surface area and porosity (per batch of three sample) $1000 N/A
Specific surface area and porosity (per sample) $380 N/A
Particle analysis instrument hire (per hour)
Malvern Mastersizer (laser diffraction) $315 $135
Malvern Zetasizer (DLS) $315 $135
Quantachrome He Pyncnometer $315 $135
Mechanical testing (per hour)
Microhardess testing - Vickers $315 $135
Nanoindentation studies (per hour, min 4 hours) $485 $1,750 per day
Tensile tester (250N) $315 $150
Rheology studies $380 $205
Thermal analysis (per sample)
Oxidation Induction Temperature $285 N/A
Low temperature DSC: - 60 to 500 ℃. $285 POA
Simultaneous thermal analysis (DSC and TGA) up to 1000 ℃ $555 POA
Simultaneous thermal analysis (DSC and TGA) above 1000 ℃ $875 POA
FTIR gas analysis (coupled to TGA/DSC) $250 N/A
Specific heat capacity $945 N/A
Thermal conductivity by laser flash (includes specific heat capacity) $1,150 POA
Coefficient of thermal expansion, Tg, sintering temperature by dilatometry $285 POA
*Training must be completed before instruments can be hired without staff assistance.

Proteomics service

We provide next-generation proteomics underpinned by data-independent acquisition (DIA) mass spectrometry and advanced computational approaches.

Overview

We use robotic sample preparation, data-independent acquisition enabled mass spectrometry and deep learning-based algorithms for rapid and simultaneous determination of the status of thousands of proteins in the sample to understand the molecular processes that are fundamental to life and wellbeing.

Details

Mass spectrometry

Instrument Capabilities and features
SCIEX TripleTOF 5600+ LC-MS/MS Mass Spectrometer High-sensitivity, high-resolution and high mass accuracy discovery and quantitation platforms. Application to qualitative (DDA) and quantitative (DIA) analysis of peptides as surrogates of proteins.
SCIEX TripleTOF 6600 LC-MS/MS Mass Spectrometer

Sample preparation

Instrument Capabilities and features
JANUS G3 liquid handling platform Automated digestion and desalting of protein samples.

Bioinformatics

Instrument Capabilities and features
High performance computing Cloud-based software tools for DDA and DIA data processing.

Using our services and equipment

Our equipment is available to staff, students, external researchers and commercial organisations at a competitive price. Our laboratory provides both self-service and full-service options. Our experienced staff can provide training, advice and guidance on the experimental process and the analysis of results, so that you achieve optimal outcomes.

Indicative pricing for external researchers (all prices exclude GST)

Service description Assisted1/CARF Drives Unassisted2/User Drives
50-200 samples (maximum 300 hours)
Sample preparation $10,300 per batch $3,000 per kit
Mass spectrometry $7,300** per year
Bioinformatics (DDA and DIA) Report for DDA included
Report for DIA negotiable
Access included
10-50 samples (maximum 75 hours)
Sample preparation $5,150 per batch $1,500 per kit
Mass spectrometry $3,650** per year
Bioinformatics (DDA and DIA) Report for DDA included
Report for DIA negotiable
Access included
1-10 samples (maximum 15 hours)
Sample preparation $1,550 per batch N/A
Mass spectrometry $155** per sample
Bioinformatics (DDA and DIA) Report for DDA included
Report for DIA negotiable
Access included

Indicative pricing for commercial organisations (all prices exclude GST)

Service description Assisted1/CARF Drives Unassisted2/User Drives
50-200 samples (maximum 300 hours)
Sample preparation $13,950 per batch $3,000 per kit
Mass spectrometry $10,950 per year
Bioinformatics (DDA and DIA) Report for DDA included
Report for DIA negotiable
Access included
10-50 samples (maximum 75 hours)
Sample preparation $6,975 per batch $1,500 per kit
Mass spectrometry $5,475 per year
Bioinformatics (DDA and DIA) Report for DDA included
Report for DIA negotiable
Access included
1-10 samples (maximum 15 hours)
Sample preparation $3,550 per batch N/A
Mass spectrometry $355 per sample
Bioinformatics (DDA and DIA) Report for DDA included
Report for DIA negotiable
Access included
** Difference between external and internal pricing covered by MOU with UQ, Griffith, USC, USQ, CQU, JCU, SCU and TRI

Terms and conditions

1 Assisted (automation service):

  • covers submitting undigested samples as a single batch up to a specified number of samples or hours (whichever is reached first) at once
  • includes protein extraction, digestion (FASP), desalting (StageTip) and mass spectrometry data acquisition by CARF staff
  • CARF induction not required
  • access to the laboratory and to the software tools not provided
  • DIA (quantitative) batches include one DDA run of PBQC (pooled) sample
  • DDA (qualitative) batches will be searched against sequences supplied by the user
  • method development (i.e. non-typical prep, targeted acquisition) and quantitative report negotiable
  • approx. turnaround time: 4-6 weeks.

2 Unassisted (instrument and cloud subscription):

  • covers submitting digested and desalted samples in multiple batches up to a specified number of samples or hours (whichever is reached first) over the period of 12 months
  • includes mass spectrometry data acquisition by CARF staff
  • user to prepare samples and analyse data (generic protocols provided)
  • subject to completion of CARF induction
  • only one user obtains access to the laboratory
  • access expires after 12 months from submitting of the first batch
  • payment is non-refundable and non-transferrable if at least one batch has already been submitted
  • includes free unlimited runs of QC-BSA and assistance with BSA data processing
  • training (i.e. in sample prep, data analysis) negotiable
  • approx. turnaround time: 2-3 weeks.

Sample preparation kit:

  • only available in combination with one of the unassisted access options
  • covers access to bench top equipment, consumables and chemicals (user to prepare all required solutions as per relevant protocols) required for protein extraction, digestion (FASP), and desalting (StageTip) over the period of 12 months
  • subject to completion of additional laboratory induction
  • access expires 12 months after submitting the first batch.

Sample preparation

We have specialist expertise in geological sample preparation and analysis, and equipment for rock cutting and crushing, core logging, thin sections and soil analysis.

Overview

Our geological rock preparation facility is based at the Banyo Pilot Plant and consists of a rock crushing and milling lab, and a geological thin section preparation lab run by specialist technicians.

Equipment

We have specialist equipment for preparing thin section samples and rock crushing.

Thin section sample preparation

Instrument Capabilities and features
Large cut-off machine with 300mm diamond blade Can section large rocks and intricate shaped rock samples
Grinding and lapping machine Accurate to 0.1 microns
Vacuum impregnation machine High-powered unit for porous samples
Grinding and polishing machine Can achieve a high-quality polish suitable for Scanning Electron Microscope (SEM) or microprobe
Diamond grinder and precision trim saw High throughput with minimal loss or defamation of the sample

Rock crushing

Instrument Capabilities and features
Jaw crusher Able to crush samples from 100mm to 5mm
Disc mill Able to mill samples from 40mm to 1mm
Ring mills with agate, tungsten carbide and steel milling heads High and low speed milling for X-ray diffraction or X-ray fluorescence
Sieve shakers Sizes from 4mm to 32 microns

Services and equipment hire

We assist students, researchers and commercial organisations to prepare geological rock samples for scientific analysis. Our services are provided on a fee-for-service basis. We will discuss your requirements and timeframes and prepare a costed proposal for your approval before commencing any work.

Indicative pricing for external researchers

Equipment, service or analysis Cost (excluding GST)
Thin section 28 x 48mm ground and cover slipped $60 per sample
Thin section 26 x 76mm ground and cover slipped $60 per sample
Thin section 50 x 76mm ground and cover slipped $115 per sample
Thin section 28 x 48mm polished $75 per sample
Thin section 26 x 76mm polished $75 per sample
Thin section 50 x 76mm polished $185 per sample
Polished epoxy mounts 25mm diameter $100 per sample
Polished epoxy mounts 30mm diameter $110 per sample
Polished epoxy mounts 40mm diameter $115 per sample
Crushing $45 per 100g sample
Milling $45 per 100g sample
Screening $45 per 100g sample

Indicative pricing for commercial organisations

Equipment, service or analysis Cost (excluding GST)
Thin sections
Thin section 28 x 48mm ground and cover slipped $100 per sample
Thin section 26 x 76mm ground and cover slipped $100 per sample
Thin section 50 x 76mm ground and cover slipped $145 per sample
Thin section 28 x 48mm polished $135 per sample
Thin section 26 x 76mm polished $135 per sample
Thin section 50 x 76mm polished $230 per sample
Extra impregnation (thin sections) $40 per sample
Blue dye impregnation $40 per sample
Mounted samples
Polished mounted samples 25mm diameter $130 per sample
Polished mounted samples 30mm diameter $150 per sample
Polished mounted samples 40mm diameter $175 per sample
Cutting - core, slabs, rock into billets $50 per sample
General sample analysis
Dry preparation of water sensitive material $45 per sample
Slabs cut, ground and sealed with epoxy $65 per sample
Gravel, sand, particles or chips into thin sections $80 per sample
Sample drying (sample received wet or damp) $25 per 100g
Sample crushing (XRD) or pulverising (XRF) $75 per 100g
Sample splitting (sub-sampling, gross sample mass greater than 100g) $75 per 100g
Sample screening/sizing (client to specify size, if required) $75 per 100g
Batch courier charge (applies to all sample crushing/splitting/screening) $55 per sample

Surface analysis

We use nanoscale imaging tools including scanning probe microscopy (SEM), transmission electron microscopy (TEM) and electron microscopy (EM).

Overview

Our laboratory houses the only helium ion microscope (HIM) and ultra-high vacuum scanning probe microscope (UHV-SPM) in Queensland. Our helium ion microscope allows researchers to visualise the outer layers of almost any solid material. Turning up the beam current allows the etching of nanoscale patterns and structures.

Details

Our state-of-the-art equipment allows for surface analysis and scanning probe microscopy.

Surface analysis

Instrument Capabilities and features
Kratos Axis Supra XPS system for automated analysis of sample surfaces, revealing elemental composition and oxidation states. The system features a monochromated Al Kα X-ray source and a helium lamp for UPS measurements on conduction bands. Samples may be sputter cleaned and depth profiled with the argon gas cluster ion source. The system also features sample heat and cool plus sensitive sample (glove box) sample transfer module.
IONTOF M6 Latest-generation ToF-SIMS instrument for high-sensitivity elemental and molecular mapping of sample surfaces. Bismuth analytical source with sub-micron spatial resolution. Argon gas-cluster ion source for depth profiling organic materials.

Scanning probe microscopy

Instrument Capabilities and features
Omicron Multiscan Lab Ultra-high Vacuum Variable Temperature Scanning Probe Microscope (UHV VT-STM) An ultra-high vacuum system supporting a wide range of surface science and nanotechnology applications. AFM and STM measurements at temperatures from 25 to 600 Kelvin, X-ray photoelectron spectroscopy (XPS), k-cell and e-beam evaporators, argon sputtering, sample preparation and growth at temperatures up to 1000°C.
Bruker Icon Dimension Multimodal AFM with ScanAsyst technology to automatically tune scan parameters in peak-force tapping mode. Quantitative mechanical property mapping (Peak-force QNM), and Kelvin Probe scanning.
Bruker Dektak Stylus profilometer for simple 1-D and 2-D measurements of sample flatness, roughness and surface feature dimensions.

Thin film characterisation

Instrument Capabilities and features
Bruker Dektak XT-A Stylus Profiler An automated stylus profilometer capable of 0.5nm repeatability. Fitted with a 2 micron radius diamond stylus and a 50mm ceramic vacuum chuck. Software allows 3D mapping, 2D stress analysis and stitching of scans up to 150mm.
JA Woollam M2000 UI Spectroscopic Ellipsometer Measures film thickness in single- and multi-layer systems, as well as refractive index and dielectric properties. Duel quartz-halogen and deuterium lamps allow spectral measurements from 245nm to 1690nm. Focusing optics for small spot analysis.

Other techniques

Instrument Capabilities and features
JA Woollam M2000UI Ellipsometer

Spectroscopic ellipsometer for determination of thin film thickness in the micron to nanometer range. Can also be used to determine the film’s optical parameters.

QSense Quartz Crystal Microbalance Quartz crystal microbalance system for adsorption and film property measurements from solution. Can be coupled to the ellipsometer for in-situ monitoring of optical properties.
Biolin Theta Flex Optical Tensiometer High precision measurement system for determining fluid contact angles and surface free energy. Cradle system allows measurement from 0-95 degrees tilt.

Services and equipment hire

Our equipment is available to staff, students, external researchers and commercial organisations at a competitive price. Our laboratory provides both self-service and full-service options.We provide sample preparation and support to suit your individual preference and budget.

Our experienced staff can provide training, advice and guidance on the experimental process and the analysis of results, so that you achieve optimal outcomes.

Indicative pricing for external researchers

Equipment, service or analysis With technician assistanceUnassisted
Atomic force microscopy (per hour) $230 $120
XPS and UPS (per hour) $230 $120
ToF-SIMS (per hour) $230 $120
Surface profilometry (per hour) $180 $80
Drop shape analysis (per hour) $180 $80
Ellipsometry (per hour) $180 $80
Quartz crystal microbalance (per day) $420 N/A
Interpretation and reporting (per hour) $100 N/A

Indicative pricing for commercial organisations

Equipment, service or analysis With technician assistance
Atomic force microscopy (per hour) $605
XPS and UPS (per hour) $605
ToF-SIMS (per hour) $605
Surface profilometry (per hour) $415
Drop shape analysis (per hour) $415
Ellipsometry (per hour) $415
Quartz crystal microbalance (per day) $900
Interpretation and reporting (per hour) $265

Synchrotron science

We can help QUT researchers access the specialist capabilities of the Australian Synchrotron, which produces X-ray and infrared radiation used in diffraction, scattering, spectroscopy and imaging experiments.

Overview

The Australian Synchrotron has unique scientific analytic capabilities with applications in Earth and environmental sciences, advanced materials, chemistry and biology.

Equipment

Beamline capabilities


The Australian Synchrotron currently has several individual experimental stations, known as beamlines, each with specialist capabilities. Current beamlines include:

Powder diffraction Powerful X-ray diffraction used to study the crystallographic structure of material
Macromolecular crystallography Single crystal X-ray diffraction to determine chemical and biological structures and analyse proteins, viruses and nucleic acids
X-ray fluorescence microscopy Elemental mapping at the micron scale
X-ray absorption spectroscopy Oxidation state and atomic coordination environment analysis suitable for chemistry, biology and materials science
Small and wide angle X-ray scattering Offers shape and size distribution from the nano- to micro-scale
Soft X-ray spectroscopy Provides surface-sensitive oxidation state and molecular arrangements
Infrared microspectroscopy High-resolution infrared microscopy
THz/Far Infrared High-resolution infrared spectroscopy

Accessing the Synchrotron

QUT researchers are able to apply to access advanced instrumentation facilities including beamline capabilities at the Australian Nuclear Science and Technology Organisation (ANSTO) and Australian Synchrotron, based in south-east Melbourne. Access to the Australian Synchrotron is free of charge for users through a peer-reviewed competitive merit system. In addition, the Australian Synchrotron provides successful proposals with travel funding and accommodation for up to three researchers.

For more information and assistance with the merit access process, please contact Research Infrastructure Specialist (Synchrotron Science), Dr Michael Jones.

Vibrational spectroscopy

We can provide chemical information on a wide range of materials using our routine and advanced infrared (FT-IR) and Raman spectrometers, microscopes and accessories.

Overview

We have a range of Fourier-transform infrared spectroscopy (FT-IR) and Raman microscopes, spectrometers and accessories, and can offer expert service and advice.

Equipment

There are two main methods used to measure molecular vibrations:

  • Infrared spectroscopy (IR or FT-IR) which involves the absorption of an infra-red photon
  • Raman spectroscopy which involves the inelastic scattering of higher energy photons.

Materials with strong IR spectra tend to give weak Raman spectra and vice versa. Thus some systems are more suited to study by one technique over the other, particularly when the different sampling requirements are taken into account.  Our expert staff can advise the most suitable method for your research.

Infrared spectroscopy

Instrument Capabilities and features
Nicolet iS50 FT-IR Spectrometer with Continuum IR microscope In built diamond ATR. Extensive searchable IR libraries. Continuum IR microscope with computerised X,Y,Z-stage. Chemical mapping to spatial resolution of 10 microns possible. Near-, Mid- and Far-IR capability, D-TGS and MCT detectors. Multiple accessories.
Nicolet iS50 FT-IR Spectrometer Emission accessory attached 100 – 1000°C. In situ chemical reaction kinetics, mineral transformations including NIR diffuse reflectance.
Nicolet Nexus 870 Step-scan spectrometer. D-TGS and MCT detectors. Near-, Mid- and Far-IR capability.
Capabilities
  • IR Microscopy, confocal, high spatial resolution in transmission mode for thin films, reflectance mode for polished surfaces or coatings on reflective surfaces (gold silver, polished metal), and micro Ge-ATR mode for polished heterogeneous or small samples, down to 10 microns.  Diamond anvil cell for even smaller quantities.
  • Extensive searchable polymer library for polymer ID (>5700 spectra), common materials and forensics (>6500 spectra) and small molecules (>9000 spectra).
  • Kinetics - monitor reactions in real time.
  • IR accessories for every sampling requirement: diamond-ATR, Ge-ATR, variable angle and polarised Ge-ATR, diffuse reflectance, specular reflectance; grazing angle specular reflectance, Imaging ATR, and photoacoustic spectroscopy.
  • IR chemical mapping of surfaces and films to 10 mm resolution, by peak or functional group or spectral decomposition methods.
  • Emission IR. Monitor reactions 100-1000°C, complement thermal measurement (DSC/TGA) by measuring the IR spectrum as a function of temperature to monitor the chemical transformations of the solid.
Raman spectroscopy
Instrument Capabilities and features
Witec Confocal Raman microscope 532 and 785 nm excitation.  Rapid chemical imaging to 0.3 µm spatial resolution.  Confocal (depth) to better than 3 µm.  High precision piezo XYZ mapping stage 200x200x20 µm.
Renishaw Qontor Raman microscope 355, 532, 633 and 785 excitation with photoluminescence capable filters. Spatial resolution <1 µm. Confocal to 5 µm. High speed encoded stage for chemical mapping. Linkham Heating (ambient to 1100°C), Heating and Cooling (77K-500K) and Oxford Microstat He (3.2K to 500K) stage.
Bruker FT-Raman spectrometer 1064nm excitation.  Low resolution XY view stage.
Capabilities
  • Raman spectroscopy with a range of excitations (355, 532, 633, 785 and 1064 µm) and an emphasis on microscopy for small or heterogeneous samples.
  • Raman imaging (chemical mapping) of surfaces and films to <-0.5 µm spatial resolution, by peak or functional group or spectral decomposition methods.
  • Mineral analysis, polymer curing, characterisation of 2D and 3D carbon (eg. graphene, diamond like films), semiconductor characterisation, inter and intramolecular interactions, and biomaterials.

Using our services and equipment

Our equipment is available to staff, students and external clients at a competitive price with both self-service and full-service options. Our experienced staff can provide training, advice and guidance on the experimental process and the analysis of results, so that you achieve optimal outcomes.

Indicative pricing for commercial organisations

Equipment, service or analysis With technician assistanceUnassisted
ATR FTIR spectra (per sample) $165 N/A

X-ray analysis

Overview

Our X-ray analysis laboratory provides X-ray Powder Diffraction (XRPD), and X-ray Fluorescence (XRF) characterisations and their related sample preparation services.

We analyse synthetic materials (for example, chemicals, steel, alloys, polymers, pharmaceuticals, nanomaterials, materials for energy applications) and natural materials (for example, soils, rocks, minerals). We support QUT researchers, collaborate with external researchers, and provide analytical consultancy to commercial organisations in the real world.

Equipment

X-ray diffraction (XRD)

X-ray diffraction (XRD) is a technique that uses the unique diffraction pattern 'fingerprint' from any solid crystalline material for qualitative identification and quantitative abundance determination of phases in mixtures of synthetic or natural materials. Typical XRD applications include qualitativeand quantitative mineralogical characterisation of mine ores, mine site remediation and road construction materials etc. Using an internal or external standard with a phase mixture also allows the quantification of the amorphous (non-diffracting) content present in the mixture. Depending on the diffraction intensity of the target phase and the mixture, working detection limits are around 1 wt.% for powder XRD.

A range of X-ray sources, optics and hardware configurations are used to optimise diffraction pattern acquisition for various analyses (for example, powder, thin film, coatings, bulk, single crystal) and phase types.

Typical XRD applications in CARF X-ray Lab include:

  • Powder diffraction (Phase ID, QPA, Lattice Parameter, Size/Strain analyses)
  • Micro-diffraction (χ-φ oscillation): mineralogical identification for precious stones and gemstones
  • Clays & Pharmaceutical samples of large d-spacings
  • Co/Fe/Mn-rich sample measurements using CrKα/ CoKα soft X-rays
  • Capillary transmission (structure solving & refinement)
  • in situ non-ambient diffraction (80K to 400K, RT to 1200℃, for Reflection & Transmission geometries)
  • GID for polycrystalline thin films & depositions on low background Si wafer
  • X-ray Reflectometry (for multi-layer thickness & interlayer roughness)
  • High Resolution XRD, Reciprocal Space Mapping for single crystal thin films (epitaxial layers)
  • Wide-Range Reciprocal Space Mapping to define single crystal orientations
  • Residual Stress (iso- & side- inclination), Texture (Pole Figure) analyses for bulk materials and polycrystalline thin films
  • Pair Distribution Function (for amorphous & crystalline powders).
Equipment
InstrumentRadiations Capabilities and features
Bruker D8 Advance XRD Cr, Co, Mo (Twist tubes) Powder diffractometer with Dynamic Beam Optimisation (Motorised Divergence Slit, Motorised Air-Scatter Knife, Dynamic Detector Opening). Parallel beam/thin film GID. 90-position sample changer; PSD (Energy Resolution <330eV) with 0/90⁰ mount; Non-ambient Chamber; Chi-Phi-Z Cradle; PolyCapillary micro-diffraction; Texture; Residual Stress; Capillary spinner.
Bruker D8 Venture SC-XRD Cu, Mo (Diamond Iμs) Large 140 × 200 mm2 without gaps PHOTON III 28 detector; Mix-mode support both photon counting and charge integration for the best sensitivity and dynamic range; Dead-time free shutterless data collection; Oxford Cryostream 1000+ (80K - 500K); Single Crystal XRD for crystal structure definition; Sample requirement: single crystal of size < 200μm; APEX5 + PROTEUM5 + SHELX software
Rigaku SmartLab XRD (ASC) Cu, Mo Powder diffractometer with parallel beam/thin film capability. 36-position sample changer; Mirrors for Divergent, Parallel, Focusing beams; Chi-phi cradle; 0D/1D/2D Pixel Area Detector; Capillary/foil Transmission; X-ray Reflectometry; Channel-cut monochromator Ge(220*2).
Rigaku SmartLab XRD (Non-ambient) Cu, Mo Powder diffractometer with parallel beam/thin film capability and small area micro-diffraction; Anton-Parr 1200⁰C oscillation stage with spinning capillary attachment; Reactor-X 1000⁰C Infrared heater; Oxford Cryostream system (down to 80K); LINKAM lowT chamber for powder reflection (down to 80K).

X-ray fluorescence (XRF)

X-ray fluorescence (XRF) is an established X-ray technique for the quantitative chemical (elemental) characterisation of samples using the characteristic X-ray emission/s of the element/s XRF can be used to characterise crystalline and non-crystalline powders, bulk samples, and liquids, including oils etc.

Sample compositions can be measured using fused glass discs, pressed powder pellets or loose powders depending on specific requirements (qualitative or quantitative data, major, minor or trace level elemental abundances).

The XRF Application packages in CARF include:

  • GEO-QUANT Advance: Turn-key solution for 21 oxides forms (Na2O, MgO, Al2O3, SiO2, P2O5, SO3, K2O, CaO, TiO2, V2O5, Cr2O3, Mn2O3, Fe2O3, NiO, CuO, ZnO, SrO, ZrO2, BaO, HfO2, PbO) common in geological samples, based on calibrations from 32x CRM, 5x QC, 3x DC
  • METAL-QUANT: Turn-key solution for 23 elements (Al, Si, P, S, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Zr, Nb, Mo, Sn, Sb, Ta, W, Pb, Bi) common in metals & alloys, with reference to 3x QC, 11x DC
  • PETRO-QUANT: Turn-key solution for 31 elements (Na, Mg, Al, Si, P, S, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Br, Zr, Mo, Ag, Cd, In, Sn, Sb, Ba, W, Tl, Pb, Bi) common in gasoline/ oil liquids, with reference to 5x QC
  • QUANT-EXPRESS: Factory calibrated semi-quantitative elemental analysis (F to U) for any unknown samples with Fundamental Parameter Modelling.

Routine suites of elements can be measured and quantified, including:

  • major and minor elements: Si, Ti, Al, Fe(III), Mn, Mg, Na, Ca, K, P, S(VI), plus Cl, F, Ba, Sr, Zr, Hf, Ni, Cu, Zn, and Pb (to nominal working detection limits of <<100ppm)
  • 41 select trace elements from Sc to U (to nominal working detection limits of 15 to <1 ppm, element dependant).

Typical XRF applications include major and trace element analysis of rocks and soils, iron and sulphide ores, mine wastes and remediation materials, cement, pigments and other components in food packaging, trace element analysis of foodstuffs, analysis of precipitates from contaminated processing water, determination of elemental ratios in nanomaterials.

Equipment
Instrument Capabilities and features
Bruker S8 Tiger Series II Wavelength Dispersive X-ray Fluorescence (WD-XRF) Spectrometer 4kW Rh tube, 7 Analysing crystals, collimators, tube filters, scintillation, gas flow detectors. 75 position auto-sample changer. Capable of analysing C to U. Routine LLD <100ppm most major elements, <1-2ppm most trace elements in rocks and soils. Automated unattended operation allowing quantitative major and trace element analysis on fused glass discs, pressed powder pellets, and qualitative elemental identification via wavelength scanning.

Sample preparation

We have access to a wide range of sample preparation equipment, including fusion apparatus, and crushing, grinding and micronising equipment. Our qualified and experienced geo-analytical staff can also provide thin section preparation and petrologic investigation capabilities. Prior to sample preparation (for example, grinding or heating) and submission, please discuss XRD and/or XRF analysis and sample requirements with our expert laboratory staff.

Equipment
Instrument Capabilities and features
McCrone Micronising Mills Reduce particle size to < 5 µm.
Rocklabs Swing Mill Reduce particle size to < 75 µm.
Claisse Le Neo Fused discs preparation for XRF.
Katanax electronic fusion instrument Preparation of inorganic samples (fusion solutions and fused disks) for X-ray fluorescence (WD-XRF and ED-XRF).
Rocklabs Swing Mills and plate crusher Rock preparation and crushing (located at Banyo Pilot Plant).
Retsch Mixer Mill Cryo-mill at LN2 temperature.
Drying ovens Drying of samples (40°C, 100°C).

Using our equipment and services

Our equipment is available to researchers and external clients at a competitive price with both full-service and self-service options. Our experienced staff can provide training, advice and guidance on the experimental process and the analysis of results, so that you achieve optimal outcomes.

Indicative pricing for external researchers

Equipment, service or analysis With technician assistance (excluding GST) Unassisted (excluding GST)
X-ray diffraction (XRD) equipment use
Bruker D8 Advance $200 per hour N/A
Rigaku Smartlab Cu/Mo - Routine 382 $200 per hour N/A
Rigaku Smartlab Cu/Mo - Research 383 $200 per hour N/A
X-ray fluorescence (XRF) services
Major elements (fused glass disc) $40 N/A
Trace elements (pressed pellet) $50 N/A

Indicative pricing for commercial organisations

Equipment, service or analysis With technician assistance (including GST) Unassisted (including GST)
X-ray diffraction (XRD) services
Quantitative powder XRD $355 per sample N/A
Quantitative powder XRD with qualitative clays $430 per sample N/A
Stand alone qualitative clay analysis $150 per sample N/A
Optional additional clay treatments (client to specify treatment/s) $55 per treatment N/A
Quantitative fine fraction XRD analysis (suitable for bulk clays and bulk respirable silica) $495 per sample N/A
X-ray instrument hire rates
X-Ray Diffractometer - high throughput Co/Cu/Mo $340 per hour $210 per hour
X-Ray Diffractometer - Cu/Mo non-ambient $400 per hour $245 per hour
X-ray fluorescence bulk sample chemical analysis
Major elements - 21 elements reported as wt% oxides, includes LOI $65 per sample N/A
Trace elements - 40 elements reported as ppm (ug/g) $65 per sample N/A

Our staff

Professor Charlotte Allen
Position
Professor
Division / Faculty
School of Earth & Atmospheric Sciences
Research fields
Geochemistry
Analytical chemistry
Geology
Email
Associate Professor Yunfei Xi
Position
Associate Professor in Materials Science
Division / Faculty
School of Chemistry & Physics
Research fields
Inorganic chemistry
Environmental management
Materials engineering
Email
Associate Professor Aaron Micallef
Position
Principal Research Infrastructure Specialist (Magnetic Resonance Spectroscopy)
Division / Faculty
Research Portfolio
Research fields
Organic chemistry
Macromolecular and materials chemistry
Physical chemistry
Email
Associate Professor Jamie Riches
Position
Principal Research Infrastructure Specialist (Electron Microscopy)
Division / Faculty
Research Portfolio
Research fields
Biochemistry and cell biology
Other physical sciences
Email
Dr David Harris
Position
Manager, Central Analytical Research Facility
Division / Faculty
Research Portfolio
Email
Dr John Caulfield
Position
Research Fellow
Division / Faculty
School of Earth & Atmospheric Sciences
Email
Dr Neven Maksemous
Position
Research Fellow
Division / Faculty
Research Portfolio
Research fields
Genetics
Neurosciences
Email
Dr Pawel Sadowski
Position
Research Fellow (Proteomics)
Division / Faculty
School of Biology & Environmental Science
Research fields
Biochemistry and cell biology
Medical biochemistry and metabolomics
Email
Dr Fernanda Zamboni
Position
Postdoctoral Research Fellow
Division / Faculty
School of Mech., Medical & Process Engineering
Email
Dr Henrietta Cathey
Position
Senior Research Officer (Electron Microprobe)
Division / Faculty
School of Earth & Atmospheric Sciences
Research field
Geology
Email
Dr Kevin Dudley
Position
Research Officer (Genomics)
Division / Faculty
School of Biology & Environmental Science
Research fields
Bioinformatics and computational biology
Agricultural biotechnology
Biochemistry and cell biology
Email
Dr Victoria Coyne
Position
Genomics Project Coordinator
Division / Faculty
Research Portfolio
Email

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